{"id":1205,"date":"2020-04-12T15:16:14","date_gmt":"2020-04-12T07:16:14","guid":{"rendered":"http:\/\/www.tangning.net\/wordpress\/?p=1205"},"modified":"2020-04-12T21:09:30","modified_gmt":"2020-04-12T13:09:30","slug":"%e6%95%99%e6%82%a8%e5%a6%82%e4%bd%95%e6%ad%a3%e7%a1%ae%e9%80%89%e6%8b%a9%e6%98%be%e5%be%ae%e9%95%9c%e7%9b%b8%e6%9c%ba%e7%9a%84%e5%88%86%e8%be%a8%e7%8e%87%e3%80%90%e8%af%91%e8%87%aa%ef%bc%9amicroscopyu","status":"publish","type":"post","link":"http:\/\/www.tangning.net\/wordpress\/?p=1205","title":{"rendered":"\u6559\u60a8\u5982\u4f55\u6b63\u786e\u9009\u62e9\u663e\u5fae\u955c\u76f8\u673a\u7684\u5206\u8fa8\u7387\u3010\u8bd1\u81ea\uff1aMicroscopyU\u3011"},"content":{"rendered":"<header class=\"articlehead\">\u4e00\u4e2a\u7535\u8377\u8026\u5408\u88c5\u7f6e\uff08\u4fd7\u79f0CCD\uff09\u6216\u4e92\u8865\u6027\u6c27\u5316\u91d1\u5c5e\u534a\u5bfc\u4f53\uff08\u4fd7\u79f0CMOS\uff09\u7684\u6210\u50cf\u82af\u7247\u7684\u6700\u7ec8\u5206\u8fa8\u7387\u53d6\u51b3\u4e8e\u5149\u7535\u4e8c\u6781\u7ba1\u7684\u6570\u91cf\u548c\u5b83\u4eec\u76f8\u5bf9\u4e8e\u663e\u5fae\u955c\u5149\u5b66\u7cfb\u7edf\u6295\u5c04\u5230\u6210\u50cf\u9635\u5217\u8868\u9762\u4e0a\u56fe\u50cf\u5c3a\u5bf8\u7684\u51fd\u6570\u6240\u51b3\u5b9a\u3002\u5f53\u5c1d\u8bd5\u5c06\u663e\u5fae\u955c\u7684\u5149\u5b66\u5206\u8fa8\u7387\u5339\u914d\u5230\u7279\u5b9a\u7684\u6570\u7801\u76f8\u673a\u548c\u89c6\u9891\u8026\u5408\u5668\u7684\u7ec4\u5408\u65f6\uff0c\u4f7f\u7528\u8be5\u8ba1\u7b97\u5668\u53ef\u786e\u5b9a\u6240\u9700\u7684\u6700\u4f4e\u50cf\u7d20\u5bc6\u5ea6\uff0c\u4ee5\u5145\u5206\u6355\u83b7\u6765\u81ea\u663e\u5fae\u955c\u7684\u6240\u6709\u5149\u5b66\u6570\u636e\u3002<\/header>\n<header class=\"articlehead\"><\/header>\n<header class=\"articlehead\"><\/header>\n<p>&nbsp;<\/p>\n<hr \/>\n<div align=\"center\"><iframe loading=\"lazy\" id=\"nikTutorial\" class=\"\" style=\"transform: scale(1);\" src=\"https:\/\/nikon.magnet.fsu.edu\/html5\/tutorials\/digitalimaging\/pixelcalculator\/mobile\/pixelcalculator.html\" width=\"600\" height=\"570\" frameborder=\"0\" marginwidth=\"0\" marginheight=\"0\" scrolling=\"no\" data-src=\"https:\/\/nikon.magnet.fsu.edu\/html5\/tutorials\/digitalimaging\/pixelcalculator\/mobile\/pixelcalculator.html\"><\/iframe><\/div>\n<hr \/>\n<p>\u8be5\u6559\u7a0b\u4ee5\u968f\u673a\u9009\u62e9\u7684\u6807\u672c\u521d\u59cb\u5316\uff0c\u663e\u793a\u5728\u201cSpecimen Image\u201d\u7a97\u53e3\uff08\u9ed1\u6846\uff09\u4e2d\uff0c\u5e76\u4ee5\u76ee\u955c\u7684\u5b54\u5f84\u6216\u6295\u5c04\u805a\u5149\u955c\u7684\u89c6\u573a\u5149\u9611\u4e3a\u754c\u3002\u6307\u5b9aCCD\u5c3a\u5bf8\uff08\u9ed8\u8ba42\/3\u82f1\u5bf8\uff09\u7684\u5f69\u8272\u77e9\u5f62\u53e0\u52a0\u5728\u56fe\u50cf\u4e0a\uff0c\u4ee5\u663e\u793a\u6210\u50cf\u82af\u7247\u6240\u6355\u83b7\u6837\u54c1\u7684\u5b9e\u9645\u533a\u57df\u3002\u5728\u6837\u672c\u56fe\u7247\u5de6\u4fa7\u7070\u8272\u3001\u9ec4\u8272\u548c\u7ea2\u8272\u7684\u65b9\u6846\u4e2d\uff0c\u56fe\u50cf\u7684\u663e\u5fae\u955c\u5149\u5b66\u5206\u8fa8\u7387\uff08\u7070\u8272\uff09\u3001CCD\u6240\u9700\u50cf\u7d20\u5c3a\u5bf8\uff08\u9ec4\u8272\uff09\u3001\u6700\u4f73CCD\u9635\u5217\u5c3a\u5bf8\uff08\u9ec4\u8272\uff09\u3001\u663e\u793a\u5668\u7684\u653e\u5927\u500d\u7387\uff08\u7ea2\u8272\uff09\u548c\u603b\u653e\u5927\u500d\u7387\uff08\u7ea2\u8272\uff09\u4ee5\u5fae\u7c73\u6216\u4e58\u79ef\u663e\u793a\uff0c\u8fd9\u4e9b\u6570\u503c\u4f1a\u968f\u7740\u4e0b\u65b9\u6ed1\u5757\u7684\u62d6\u52a8\u800c\u6301\u7eed\u66f4\u65b0\u3002\u901a\u8fc7\u6837\u672c\u56fe\u7247\u4e0b\u65b9\u7684\u5355\u9009\u6309\u94ae\uff0c\u53ef\u4ee5\u9009\u62e9\u65b0\u7684CCD\u683c\u5f0f\uff08\u5c3a\u5bf8\uff09\u3002\u6240\u9009\u4f20\u611f\u5668\u7684\u7269\u7406CCD\u5c3a\u5bf8\uff08\u4ee5\u6beb\u7c73\u4e3a\u5355\u4f4d\uff09\u6cbf\u7740\u4e0e\u6210\u50cf\u82af\u7247\u5177\u6709\u76f8\u540c\u957f\u5bbd\u6bd4\u7684\u77e9\u5f62\u4e00\u8d77\u663e\u793a\u5728\u6837\u672c\u56fe\u7247\u7684\u53f3\u4fa7\u3002<\/p>\n<p>&nbsp;<\/p>\n<p>\u64cd\u4f5c\u8be5\u6559\u7a0b\uff0c\u8bf7\u79fb\u52a8\u6570\u503c\u5b54\u5f84\uff08Numerical Aperture\uff09\u548c\u7269\u955c\u653e\u5927\u500d\u7387\uff08Objective Magnification\uff09\u6ed1\u5757\uff0c\u4ee5\u4e3a\u9700\u8981\u8003\u8651\u7684\u663e\u5fae\u955c\u5149\u5b66\u914d\u7f6e\u8bbe\u7f6e\u9002\u5f53\u7684\u6570\u503c\u3002\u63a5\u7740\uff0c\u9009\u62e9\u4e00\u4e2a\u76ee\u955c\u6216\u6295\u5c04\u805a\u5149\u955c\u7684\u89c6\u91ce\u6570\uff08\u4ece18\uff5e26\u6beb\u7c73\uff09\u548c\u89c6\u9891\u8026\u5408\u5668\uff08\u4e2d\u7ee7\u955c\uff09\u7684\u653e\u5927\u500d\u7387\uff08\u4ece0.5x\uff5e1.0x\u4e4b\u95f4\uff09\u3002\u8026\u5408\u5668\u6ed1\u5757\u88ab\u79fb\u52a8\u65f6\uff0c\u53e0\u52a0\u5728\u6837\u672c\u56fe\u7247\u4e0a\u7684\u77e9\u5f62\u7684\u5927\u5c0f\u968f\u4e4b\u6539\u53d8\uff0c\u4ee5\u5339\u914dCCD\u4f20\u611f\u5668\u6240\u6355\u83b7\u7684\u6837\u672c\u533a\u57df\u3002\u53ef\u4ee5\u7528\u201cChoose a Specimen\u201d\u4e0b\u62c9\u83dc\u5355\u968f\u65f6\u9009\u62e9\u4e00\u4e2a\u65b0\u7684\u6807\u672c\u3002<\/p>\n<p>&nbsp;<\/p>\n<p>\u5c06\u5149\u5b66\u663e\u5fae\u955c\u4ea7\u751f\u7684\u56fe\u50cf\u6355\u83b7\u81f3CCD\u6216CMOS\u56fe\u50cf\u4f20\u611f\u5668\u4e0a\u7684\u5149\u7535\u4e8c\u6781\u7ba1\u9635\u5217\u7684\u6548\u7387\u53d6\u51b3\u4e8e\u591a\u4e2a\u56e0\u7d20\uff0c\u4ece\u7269\u955c\u653e\u5927\u500d\u7387\u3001\u6570\u503c\u5b54\u5f84\u548c\u5206\u8fa8\u7387\u3001\u7535\u5b50\u56fe\u50cf\u4f20\u611f\u5668\u5149\u7535\u4e8c\u6781\u7ba1\u9635\u5217\u7684\u5c3a\u5bf8\u3001\u957f\u5bbd\u6bd4\u3001\u6444\u50cf\u4e2d\u7ee7\u955c\u500d\u7387\uff0c\u4ee5\u53ca\u9635\u5217\u4e2d\u5404\u4e2a\u5149\u611f\u5143\u4ef6\u7684\u5c3a\u5bf8\u3002\u6b64\u5916\uff0c\u8fd8\u5fc5\u987b\u8003\u8651\u7528\u4e8e\u6210\u50cf\u7684\u7279\u5b9a\u6837\u672c\u7684\u53c2\u6570\uff0c\u4f8b\u5982\u5bf9\u6bd4\u5ea6\u3001\u4fe1\u566a\u6bd4\u3001\u573a\u666f\u5185\u52a8\u6001\u8303\u56f4\u548c\u79ef\u5206\u65f6\u95f4\u3002<\/p>\n<p>&nbsp;<\/p>\n<p>CCD\u6700\u7ec8\u7684\u5149\u5b66\u5206\u8fa8\u7387\u53d6\u51b3\u4e8e\u5149\u7535\u4e8c\u6781\u7ba1\u7684\u6570\u91cf\u548c\u5b83\u4eec\u76f8\u5bf9\u4e8e\u663e\u5fae\u955c\u5149\u5b66\u900f\u955c\u7cfb\u7edf\u6295\u5c04\u5230\u6210\u50cf\u9635\u5217\u8868\u9762\u4e0a\u56fe\u50cf\u5c3a\u5bf8\u7684\u51fd\u6570\u6240\u51b3\u5b9a\uff0c\u5f53\u524d\u53ef\u7528\u7684CCD\u9635\u5217\u968f\u7740\u5c3a\u5bf8\u7684\u53d8\u5316\uff0c\u4ece\u51e0\u767e\u81f3\u6570\u5343\u50cf\u7d20\u3002\u7528\u4e8e\u79d1\u7814\u8bbe\u5907\u4e2d\u7684\u73b0\u4ee3\u9635\u5217\u5c3a\u5bf8\u8303\u56f4\u4ece1000\u00d71000\u52305000\u00d75000\u4e2a\u4f20\u611f\u5668\u5143\u4ef6\u3002\u6d88\u8d39\u7ea7\u548c\u79d1\u5b66\u7ea7CCD\u7684\u53d1\u5c55\u8d8b\u52bf\u662f\u4f20\u611f\u5668\u5c3a\u5bf8\u6301\u7eed\u51cf\u5c0f\uff0c\u76ee\u524d\uff0c\u5177\u6709\u5c0f\u81f34\u00d74\u5fae\u7c73\u5149\u7535\u4e8c\u6781\u7ba1\u7684\u6570\u7801\u76f8\u673a\u5df2\u7ecf\u9762\u5e02\u3002<\/p>\n<p>&nbsp;<\/p>\n<p>\u53ea\u6709\u4e3a\u6bcf\u4e2a\u53ef\u5206\u8fa8\u5355\u5143\u5236\u4f5c\u81f3\u5c11\u4e24\u4e2a\u6837\u672c\uff0c\u624d\u80fd\u5b9e\u73b0\u7528\u663e\u5fae\u955c\u7684\u5149\u5b66\u5143\u4ef6\u5bf9\u6837\u54c1\u6210\u50cf\u7684\u8db3\u591f\u7684\u5206\u8fa8\u7387\uff0c\u5c3d\u7ba1\u8bb8\u591a\u7814\u7a76\u4eba\u5458\u66f4\u613f\u610f\u4e3a\u6bcf\u4e2a\u53ef\u5206\u8fa8\u5355\u5143\u63d0\u4f9b\u4e09\u4e2a\u6837\u672c\u4ee5\u786e\u4fdd\u8db3\u591f\u7684\u91c7\u6837\u3002\u5728\u8bf8\u5982\u663e\u5fae\u955c\u8fd9\u4e00\u7c7b\u53d7\u9650\u4e8e\u884d\u5c04\u7684\u5149\u5b66\u4eea\u5668\u4e2d\uff0c\u5f53\u4f7f\u7528\u6570\u503c\u5b54\u5f84\u4e3a1.4\u7684\u7269\u955c\u65f6\uff0c\u5728\u5e73\u5747\u53ef\u89c1\u5149\u6ce2\u957f\uff08550\u7eb3\u7c73\uff09\u5904\u7684\u5149\u5b66\u5206\u8fa8\u7387\u7684\u963f\u8d1d\u6781\u9650\u4e3a0.2\u5fae\u7c73\u3002\u5728\u8fd9\u79cd\u60c5\u51b5\u4e0b\uff0c\u9996\u90097\u00d77\u5fae\u7c73\u7684\u4f20\u611f\u5668\u5c3a\u5bf8\uff0c10\u5e73\u65b9\u5fae\u7c73\u7684\u4f20\u611f\u5668\u5c3a\u5bf8\u521a\u597d\u8db3\u591f\u5927\uff0c\u4ee5\u8ba9\u5149\u5b66\u548c\u7535\u5b50\u5206\u8fa8\u7387\u8fdb\u884c\u5339\u914d\u3002\u5c3d\u7ba1CCD\u6210\u50cf\u4f20\u611f\u5668\u4e2d\u66f4\u5c0f\u7684\u5149\u7535\u4e8c\u6781\u7ba1\u53ef\u4ee5\u63d0\u9ad8\u7a7a\u95f4\u5206\u8fa8\u7387\uff0c\u4f46\u540c\u65f6\u5b83\u4eec\u4e5f\u9650\u5236\u4e86\u8bbe\u5907\u7684\u52a8\u6001\u8303\u56f4\u3002<\/p>\n<p>&nbsp;<\/p>\n<hr \/>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-1213 aligncenter\" src=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/04\/WX20200412-175108.png\" alt=\"\" width=\"515\" height=\"1025\" srcset=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/04\/WX20200412-175108.png 515w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/04\/WX20200412-175108-151x300.png 151w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/04\/WX20200412-175108-514x1024.png 514w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/04\/WX20200412-175108-301x600.png 301w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/04\/WX20200412-175108-80x160.png 80w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/04\/WX20200412-175108-100x200.png 100w\" sizes=\"auto, (max-width: 515px) 100vw, 515px\" \/><\/p>\n<p style=\"text-align: center;\"><strong>\u8868\u4e00\uff1a\u5339\u914d\u663e\u5fae\u955c\u5149\u5b66\u5206\u8fa8\u7387\u6240\u9700\u7684\u50cf\u7d20\u5c3a\u5bf8<\/strong><\/p>\n<hr 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\/>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-1212 aligncenter\" src=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/04\/WX20200412-175156.png\" alt=\"\" width=\"99\" height=\"49\" \/><\/p>\n<hr \/>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-1211 aligncenter\" src=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/04\/WX20200412-175224.png\" alt=\"\" width=\"128\" height=\"54\" \/><\/p>\n<hr \/>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-1210 aligncenter\" src=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/04\/WX20200412-175250.png\" alt=\"\" width=\"234\" height=\"59\" \/><\/p>\n<hr \/>\n<p>&nbsp;<\/p>\n<p>\u5176\u4e2d r \u662f\u5206\u8fa8\u7387\uff08\u4e24\u4e2a\u6837\u672c\u70b9\u4e4b\u95f4\u7684\u6700\u5c0f\u53ef\u5206\u8fa8\u8ddd\u79bb\uff09\uff0cNA \u7b49\u4e8e\u7269\u955c\u7684\u6570\u503c\u5b54\u5f84\uff0c\u03bb 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class=\"textblock intro\">\n<p>\u64b0\u7a3f\u4eba\uff1a<\/p>\n<\/div>\n<div id=\"footnotes\">\n<p><strong>Matthew J. Parry-Hill, Kimberly M. Vogt, John D. Griffin, and Michael W. Davidson &#8211; National High Magnetic Field Laboratory, 1800 East Paul Dirac Dr., The Florida State University, Tallahassee, Florida, 32310.<\/strong><\/p>\n<\/div>\n<div id=\"nii-links\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>\u4e00\u4e2a\u7535\u8377\u8026\u5408\u88c5\u7f6e\uff08\u4fd7\u79f0CCD\uff09\u6216\u4e92\u8865\u6027\u6c27\u5316\u91d1\u5c5e\u534a\u5bfc\u4f53\uff08\u4fd7\u79f0CMOS\uff09\u7684\u6210\u50cf\u82af\u7247\u7684\u6700\u7ec8\u5206\u8fa8\u7387\u53d6\u51b3\u4e8e\u5149\u7535 &hellip;<\/p>\n","protected":false},"author":1,"featured_media":1209,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[17,18],"tags":[],"class_list":["post-1205","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-microscopyu","category-tutorials-on-microscopyu"],"_links":{"self":[{"href":"http:\/\/www.tangning.net\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/1205","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/www.tangning.net\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.tangning.net\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.tangning.net\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.tangning.net\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1205"}],"version-history":[{"count":9,"href":"http:\/\/www.tangning.net\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/1205\/revisions"}],"predecessor-version":[{"id":1219,"href":"http:\/\/www.tangning.net\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/1205\/revisions\/1219"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/www.tangning.net\/wordpress\/index.php?rest_route=\/wp\/v2\/media\/1209"}],"wp:attachment":[{"href":"http:\/\/www.tangning.net\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1205"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.tangning.net\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=1205"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.tangning.net\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=1205"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}