{"id":1177,"date":"2020-03-15T18:37:25","date_gmt":"2020-03-15T10:37:25","guid":{"rendered":"http:\/\/www.tangning.net\/wordpress\/?p=1177"},"modified":"2020-03-16T07:07:28","modified_gmt":"2020-03-15T23:07:28","slug":"%e4%b8%ba%e8%8d%a7%e5%85%89%e6%8e%a2%e9%92%88%e5%8c%b9%e9%85%8d%e5%b0%bc%e5%ba%b7%e8%8d%a7%e5%85%89%e6%bb%a4%e5%85%89%e5%9d%97%e3%80%90%e8%af%91%e8%87%aa%ef%bc%9amicroscopyu%e3%80%91","status":"publish","type":"post","link":"http:\/\/www.tangning.net\/wordpress\/?p=1177","title":{"rendered":"\u4e3a\u8367\u5149\u63a2\u9488\u5339\u914d\u5c3c\u5eb7\u8367\u5149\u6ee4\u5149\u5757\u3010\u8bd1\u81ea\uff1aMicroscopyU\u3011"},"content":{"rendered":"<header class=\"articlehead\">\u73b0\u4ee3\u8367\u5149\u663e\u5fae\u955c\u4eea\u5668\u7ed3\u5408\u4e86\u5e72\u6d89\u6ee4\u5149\u7247\u548c\u53cc\u8272\u5206\u5149\u955c\u7247\uff0c\u4ee5\u6ee1\u8db3\u7528\u4e8e\u6807\u8bb0\u6837\u672c\u7684\u8367\u5149\u63a2\u9488\u7684\u6fc0\u53d1\u548c\u53d1\u5c04\u7684\u8981\u6c42\u3002\u5982\u679c\u8fd9\u4e9b\u7ec4\u4ef6\u9009\u62e9\u6070\u5f53\uff0c\u663e\u5fae\u955c\u5c06\u4e3a\u6837\u672c\u8367\u5149\u56e2\u7684\u9009\u62e9\u6027\u6fc0\u53d1\uff0c\u4ee5\u53ca\u968f\u540e\u5206\u79bb\u51fa\u6210\u50cf\u6240\u5fc5\u987b\u7684\u5f31\u5f97\u591a\u7684\u53d1\u5c04\u8367\u5149\u63d0\u4f9b\u4e86\u4e00\u79cd\u5fc5\u4e0d\u53ef\u5c11\u7684\u673a\u5236\u3002\u4ed4\u7ec6\u7684\u5c06\u6fc0\u53d1\u548c\u53d1\u5c04\u6ee4\u7247\u7684\u5c5e\u6027\uff0c\u4ee5\u53ca\u4e8c\u5411\u8272\u955c\u7684\u529f\u80fd\u4e0e\u88ab\u6807\u8bb0\u6837\u672c\u7684\u7279\u5f81\u76f8\u5339\u914d\uff0c\u53ef\u4ee5\u5728\u9ed1\u8272\u80cc\u666f\u4e0a\u4ee5\u6700\u5927\u7684\u654f\u611f\u5ea6\u8fdb\u884c\u6210\u50cf\u3002\u8be5\u4ea4\u4e92\u5f0f\u6559\u7a0b\u4f7f\u7528\u6237\u53ef\u4ee5\u5728\u5f53\u524d\u5c3c\u5eb7\u7684\u8367\u5149\u6ee4\u5149\u5757\u4e2d\u786e\u5b9a\u6700\u4f73\u7684\u9009\u62e9\uff0c\u4ee5\u6700\u5927\u5316\u7279\u5b9a\u8367\u5149\u63a2\u9488\u7684\u6fc0\u53d1\u548c\u53d1\u5c04\u6548\u7387\u3002<\/header>\n<header class=\"articlehead\"><\/header>\n<p>&nbsp;<\/p>\n<div align=\"center\"><iframe loading=\"lazy\" id=\"nikTutorial\" class=\"\" style=\"transform: scale(1);\" src=\"https:\/\/nikon.magnet.fsu.edu\/html5\/tutorials\/spectralprofiles\/mobile\/spectralprofiles.html\" width=\"580\" height=\"600\" frameborder=\"0\" marginwidth=\"0\" marginheight=\"0\" scrolling=\"no\" data-src=\"https:\/\/nikon.magnet.fsu.edu\/html5\/tutorials\/spectralprofiles\/mobile\/spectralprofiles.html\"><\/iframe><\/div>\n<p>&nbsp;<\/p>\n<p>\u64cd\u4f5c\u8be5\u6559\u7a0b\uff0c\u9996\u5148\u4ece\u7a97\u53e3\u53f3\u4fa7\u7684\u201cNikon Filter Set\u201d\u4e0b\u62c9\u83dc\u5355\u4e2d\u9009\u62e9\u4e00\u7ec4\u6ee4\u5149\u5757\u3002\u63a5\u7740\u4ece\u201cProbe Class\u201d\u4e0b\u62c9\u83dc\u5355\u4e2d\u9009\u62e9\u6240\u7528\u8367\u5149\u67d3\u6599\u7684\u65cf\u7c7b\u3002\u5bf9\u4e8e\u6bcf\u4e00\u7c7b\u63a2\u9488\uff0c\u90fd\u63d0\u4f9b\u4e86\u4e00\u7ec4\u8367\u5149\u56e2\uff0c\u53ef\u4ee5\u901a\u8fc7\u201cFluorophore\u201d\u7684\u4e0b\u62c9\u83dc\u5355\u8fdb\u884c\u8bbf\u95ee\u3002\uff08\u5c0f\u8d34\u58eb\uff1a\u4e3a\u4e86\u66f4\u5feb\u6377\u7684\u64cd\u4f5c\uff0c\u5728\u9009\u62e9\u6240\u9700\u7684\u8367\u5149\u63a2\u9488\u540e\uff0c\u53ef\u4ee5\u901a\u8fc7\u5411\u4e0a\u548c\u5411\u4e0b\u7684\u7bad\u5934\u952e\u5feb\u901f\u5207\u6362\u6ee4\u5149\u5757\u7684\u9009\u62e9\u3002\uff09<\/p>\n<p>&nbsp;<\/p>\n<p>\u8be5\u6559\u7a0b\u4ee5\u84dd\u5149\u5438\u6536\u7684\u8367\u5149\u63a2\u9488\uff08Alexa Fluor 488\uff09\u7684\u5149\u8c31\u56fe\u8fdb\u884c\u521d\u59cb\u5316\uff0c\u53e0\u52a0\u4e0a\u5339\u914d\u7684\u4e00\u7ec4\u5c3c\u5eb7\u6ee4\u5149\u5757\u7684\u5149\u8c31\u7279\u6027\uff0c\u6700\u7ec8\u663e\u793a\u5728\u6807\u9898\u4e3a\u201cSpectral Profiles\u201d\u7684\u56fe\u50cf\u4e2d\u3002\u6240\u9009\u7684\u8367\u5149\u56e2\u7684\u6fc0\u53d1\u548c\u53d1\u5c04\u5149\u8c31\u7684\u6807\u51c6\u5316\u5f3a\u5ea6\u4ee5\u56fe\u50cf\u53f3\u4fa7\u7684\u7eb5\u5750\u6807\uff08Relative Intensity\uff09\u6807\u793a\uff0c\u800c\u5de6\u4fa7\u7684\u7eb5\u5750\u6807\u5219\u6807\u793a\u6240\u9009\u6ee4\u5149\u7247\u7ec4\u5404\u90e8\u4ef6\u7684\u5149\u8c31\u900f\u8fc7\u7387\uff08Transmittance\uff09\u3002\u5149\u8c31\u622a\u9762\u7531\u84dd\u8272\u548c\u6d45\u7ea2\u8272\u9634\u5f71\u5206\u522b\u6807\u8bc6\u6fc0\u53d1\u548c\u53d1\u5c04\u7684\u91cd\u53e0\u533a\u57df\u3002\u6fc0\u53d1\u622a\u9762\uff08\u84dd\u8272\uff09\u5bf9\u5e94\u8367\u5149\u56e2\u7684\u5438\u6536\u548c\u6fc0\u53d1\u6ee4\u5149\u7247\u900f\u8fc7\u533a\u57df\u7684\u91cd\u53e0\uff0c\u53d1\u5c04\u622a\u9762\uff08\u7ea2\u8272\uff09\u5219\u8868\u793a\u8367\u5149\u56e2\u7684\u53d1\u5c04\u548c\u53d1\u5c04\u6ee4\u7247\u900f\u8fc7\u533a\u57df\u7684\u91cd\u53e0\u3002\u6bcf\u4e00\u4e2a\u622a\u9762\u7684\u91cd\u53e0\u7740\u8272\u90fd\u53ef\u4ee5\u901a\u8fc7\u201cExcitation\u201d\u548c\u201cEmission\u201d\u590d\u9009\u6846\u9010\u4e2a\u6253\u5f00\u6216\u5173\u95ed\u3002\u5f53\u201cFluorophore Spectra\u201d\u4e2d\u7684\u590d\u9009\u6846\u88ab\u5173\u95ed\uff0c\u540c\u6837\u4f1a\u53bb\u9664\u8367\u5149\u56e2\u7684\u201cAbsorption\u201d \u548c\/\u6216\u201cEmission\u201d\u76f8\u5e94\u7684\u989c\u8272\u9634\u5f71\u3002\u6ee4\u5149\u5757\u6bcf\u4e00\u90e8\u4ef6\u7684\u5149\u8c31\u540c\u6837\u53ef\u4ee5\u901a\u8fc7\u201cFilter\u00a0 Spectra\u201d\u590d\u9009\u6846\u4e2d\u7684\u590d\u9009\u6807\u8bb0\u5206\u522b\u79fb\u9664\u3002\u6fc0\u53d1\u7247\u3001\u4e8c\u5411\u8272\u955c\u3001\u53d1\u5c04\u7247\u7684\u900f\u5c04\u66f2\u7ebf\u88ab\u5206\u522b\u7528\u84dd\u8272\u3001\u7eff\u8272\u548c\u7ea2\u8272\u8fdb\u884c\u989c\u8272\u7f16\u7801\uff0c\u5e76\u53ef\u4ee5\u4f7f\u7528\u76f8\u5e94\u7684\u590d\u9009\u6846\u5207\u6362\u5f00\u6216\u5173\u3002\u8bf7\u6ce8\u610f\uff0c\u91cd\u8981\u7684\u6ee4\u5149\u7247\u5149\u8c31\u53c2\u6570\u548c\u7528\u4e8e\u8367\u5149\u63a2\u9488\u6fc0\u53d1\u548c\u53d1\u5c04\u7684\u6ce2\u957f\u5cf0\u503c\u4e5f\u663e\u793a\u5728\u6ee4\u5149\u7247\u548c\u63a2\u9488\u7684\u590d\u9009\u6846\u65c1\u8fb9\u3002<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<div class=\"textblock intro\">\n<p>\u64b0\u7a3f\u4eba\uff1a<\/p>\n<\/div>\n<div id=\"footnotes\">\n<p><strong>Adam Rainey and Michael W. Davidson &#8211; National High Magnetic Field Laboratory, 1800 East Paul Dirac Dr., The Florida State University, Tallahassee, Florida, 32310.<\/strong><\/p>\n<\/div>\n<div id=\"nii-links\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>\u73b0\u4ee3\u8367\u5149\u663e\u5fae\u955c\u4eea\u5668\u7ed3\u5408\u4e86\u5e72\u6d89\u6ee4\u5149\u7247\u548c\u53cc\u8272\u5206\u5149\u955c\u7247\uff0c\u4ee5\u6ee1\u8db3\u7528\u4e8e\u6807\u8bb0\u6837\u672c\u7684\u8367\u5149\u63a2\u9488\u7684\u6fc0\u53d1\u548c\u53d1\u5c04\u7684\u8981\u6c42\u3002\u5982 &hellip;<\/p>\n","protected":false},"author":1,"featured_media":1150,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[17,18],"tags":[],"class_list":["post-1177","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-microscopyu","category-tutorials-on-microscopyu"],"_links":{"self":[{"href":"http:\/\/www.tangning.net\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/1177","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/www.tangning.net\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.tangning.net\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.tangning.net\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.tangning.net\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1177"}],"version-history":[{"count":3,"href":"http:\/\/www.tangning.net\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/1177\/revisions"}],"predecessor-version":[{"id":1180,"href":"http:\/\/www.tangning.net\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/1177\/revisions\/1180"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/www.tangning.net\/wordpress\/index.php?rest_route=\/wp\/v2\/media\/1150"}],"wp:attachment":[{"href":"http:\/\/www.tangning.net\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1177"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.tangning.net\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=1177"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.tangning.net\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=1177"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}