{"id":1159,"date":"2020-03-10T14:49:57","date_gmt":"2020-03-10T06:49:57","guid":{"rendered":"http:\/\/www.tangning.net\/wordpress\/?p=1159"},"modified":"2020-03-15T17:31:33","modified_gmt":"2020-03-15T09:31:33","slug":"%e8%a7%86%e9%87%8e%e6%b7%b1%e5%ba%a6%e7%9a%84%e8%ae%a1%e7%ae%97%e3%80%90%e8%bd%ac%e8%87%aa%ef%bc%9amicroscopyu%e3%80%91","status":"publish","type":"post","link":"http:\/\/www.tangning.net\/wordpress\/?p=1159","title":{"rendered":"\u89c6\u91ce\u6df1\u5ea6\u7684\u8ba1\u7b97\u3010\u8bd1\u81ea\uff1aMicroscopyU\u3011"},"content":{"rendered":"<header class=\"articlehead\">\u8be5\u4ea4\u4e92\u5f0f\u6559\u7a0b\u5c06\u5e2e\u52a9\u60a8\u63a2\u7d22\u5c3c\u5eb7\u5e38\u89c1\u7269\u955c\u7684\u8fd1\u4f3c\u89c6\u91ce\u6df1\u5ea6\u3002\u7528\u6237\u53ef\u4ee5\u901a\u8fc7\u4e0b\u62c9\u83dc\u5355\u6765\u9009\u62e9\u5c3c\u5eb7\u73b0\u6709CFI60\u65e0\u9650\u8fdc\u5149\u5b66\u77eb\u6b63\u7269\u955c\u9635\u5bb9\u4e2d\u4efb\u610f\u4e00\u9897\u7269\u955c\uff0c\u7528\u4f5c\u76f8\u5dee\u6821\u51c6\u51fd\u6570\u7684\u8fd0\u7b97\u3002<\/header>\n<header class=\"articlehead\"><\/header>\n<p>&nbsp;<\/p>\n<div align=\"center\"><iframe loading=\"lazy\" id=\"nikTutorial\" class=\"\" style=\"transform: scale(1);\" src=\"https:\/\/nikon.magnet.fsu.edu\/html5\/tutorials\/depthoffield\/mobile\/depthoffield.html\" width=\"450\" height=\"230\" frameborder=\"0\" marginwidth=\"0\" marginheight=\"0\" scrolling=\"no\" data-src=\"https:\/\/nikon.magnet.fsu.edu\/html5\/tutorials\/depthoffield\/mobile\/depthoffield.html\"><\/iframe><\/div>\n<p>&nbsp;<\/p>\n<p>\u64cd\u4f5c\u8be5\u6559\u7a0b\uff0c\u9996\u5148\u4ece\u201cChoose an Objective\u201d\u4e0b\u62c9\u83dc\u5355\u4e2d\u9009\u62e9\u4e00\u9897\u5408\u9002\u7684\u7269\u955c\u3002\u4e0b\u62c9\u83dc\u5355\u7684\u5217\u8868\u4e2d\u6db5\u76d6\u4e86\u5c3c\u5eb7\u73b0\u6709\u7684\u6d88\u8272\u5dee\u3001\u5e73\u573a\u6d88\u8272\u5dee\u3001\u5e73\u573a\u8424\u77f3\u53ca\u5e73\u573a\u590d\u6d88\u8272\u5dee\u7cfb\u5217\u7684\u7269\u955c\uff0c\u4e00\u4e9b\u4e13\u7528\u7269\u955c\u4e5f\u5305\u542b\u5728\u5185\u3002\u7b2c\u4e8c\u6b65\uff0c\u4f7f\u7528\u201cChoose 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loading=\"lazy\" decoding=\"async\" class=\"wp-image-1163 aligncenter\" src=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/03\/WX20200310-144912.png\" alt=\"\" width=\"397\" height=\"135\" srcset=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/03\/WX20200310-144912.png 559w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/03\/WX20200310-144912-300x102.png 300w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/03\/WX20200310-144912-280x95.png 280w\" sizes=\"auto, (max-width: 397px) 100vw, 397px\" \/><\/p>\n<p>&nbsp;<\/p>\n<div class=\"textblock intro\">\n<p>\u7528 d(tot) \u6765\u8868\u793a\u89c6\u91ce\u6df1\u5ea6\uff0c<img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-1164\" src=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/03\/WX20200310-154149.png\" alt=\"\" width=\"12\" height=\"16\" srcset=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/03\/WX20200310-154149.png 124w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/03\/WX20200310-154149-120x160.png 120w\" sizes=\"auto, (max-width: 12px) 100vw, 12px\" \/>\u00a0\u662f\u7167\u660e\u5149\u7684\u6ce2\u957f\uff0cn\u662f\u76d6\u73bb\u7247\u4e0e\u7269\u955c\u524d\u7aef\u955c\u7247\u4e4b\u95f4\u4ecb\u8d28\u7684\u6298\u5c04\u7387\uff08\u901a\u5e38\u7a7a\u6c14\u4e3a1.000\uff0c\u6d78\u5165\u955c\u6cb9\u4e3a1.515\uff09\uff0cNA\u7b49\u4e8e\u7269\u955c\u7684\u6570\u503c\u5b54\u5f84\u3002\u53d8\u91cfe\u662f\u7531\u653e\u7f6e\u5728\u663e\u5fae\u955c\u7269\u955c\u6210\u50cf\u9762\u7684\u63a2\u6d4b\u5668\u6240\u51b3\u5b9a\u7684\u6700\u5c0f\u8ddd\u79bb\u3002M\u662f\u6a2a\u5411\u653e\u5927\u500d\u7387\u3002\u4f7f\u7528\u8be5\u516c\u5f0f\uff0c\u89c6\u91ce\u6df1\u5ea6 d(tot) \u548c\u6ce2\u957f\u00a0<img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-1164\" src=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/03\/WX20200310-154149.png\" alt=\"\" width=\"12\" height=\"16\" srcset=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/03\/WX20200310-154149.png 124w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/03\/WX20200310-154149-120x160.png 120w\" sizes=\"auto, (max-width: 12px) 100vw, 12px\" \/>\u00a0\u5fc5\u987b\u8868\u8fbe\u4e3a\u76f8\u540c\u7684\u5355\u4f4d\u3002\u4f8b\u5982\uff0c\u5982\u679c d(tot) \u88ab\u8ba1\u7b97\u4e3aum\u7684\u957f\u5ea6\u5355\u4f4d\uff0c\u5219\u00a0<img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-1164\" src=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/03\/WX20200310-154149.png\" alt=\"\" width=\"12\" height=\"16\" srcset=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/03\/WX20200310-154149.png 124w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/03\/WX20200310-154149-120x160.png 120w\" sizes=\"auto, (max-width: 12px) 100vw, 12px\" \/>\u00a0\u4e5f\u5fc5\u987b\u4ee5um\u4e3a\u5355\u4f4d\u4ee3\u5165\u516c\u5f0f\u4e2d\uff08700nm\u7684\u7ea2\u5149\u8981\u4ee50.7um\u7684\u5f62\u5f0f\u5199\u5165\u516c\u5f0f\uff09\u3002\u6ce8\u610f\u5230\u89c6\u91ce\u7684\u884d\u5c04\u6781\u9650\u6df1\u5ea6\uff08\u516c\u5f0f\u7b2c\u4e00\u9879\uff09\u4ee5\u6570\u503c\u5b54\u5f84\u7684\u5e73\u65b9\u53cd\u5411\u6536\u7f29\uff0c\u800c\u6a2a\u5411\u5206\u8fa8\u7387\u6781\u9650\u5219\u4ee5\u4e0e\u6570\u503c\u5b54\u5f84\u4e00\u6b21\u5e42\u6210\u53cd\u6bd4\u7684\u5f62\u5f0f\u7f29\u5c0f\u3002\u56e0\u6b64\uff0c\u8f74\u5411\u5206\u8fa8\u7387\u548c\u5149\u5b66\u5207\u7247\u6240\u80fd\u5230\u8fbe\u7684\u539a\u5ea6\u53d7\u7cfb\u7edf\u6570\u503c\u5b54\u5f84\u7684\u5f71\u54cd\u8fdc\u5927\u4e8e\u663e\u5fae\u955c\u7684\u6a2a\u5411\u5206\u8fa8\u7387\u3002<\/p>\n<\/div>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<div class=\"textblock intro\">\n<p>\u64b0\u7a3f\u4eba\uff1a<\/p>\n<\/div>\n<div id=\"footnotes\">\n<p><strong>Kenneth R. Spring<\/strong> &#8211; Scientific Consultant, Lusby, Maryland, 20657.<\/p>\n<p><strong>John C. Long and Michael W. Davidson<\/strong> &#8211; National High Magnetic Field Laboratory, 1800 East Paul Dirac Dr., The Florida State University, Tallahassee, Florida, 32310.<\/p>\n<\/div>\n<div id=\"nii-links\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>\u8be5\u4ea4\u4e92\u5f0f\u6559\u7a0b\u5c06\u5e2e\u52a9\u60a8\u63a2\u7d22\u5c3c\u5eb7\u5e38\u89c1\u7269\u955c\u7684\u8fd1\u4f3c\u89c6\u91ce\u6df1\u5ea6\u3002\u7528\u6237\u53ef\u4ee5\u901a\u8fc7\u4e0b\u62c9\u83dc\u5355\u6765\u9009\u62e9\u5c3c\u5eb7\u73b0\u6709CFI60\u65e0\u9650 &hellip;<\/p>\n","protected":false},"author":1,"featured_media":1152,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[18],"tags":[],"class_list":["post-1159","post","type-post","status-publish","format-standard","has-post-thumbnail","hentry","category-tutorials-on-microscopyu"],"_links":{"self":[{"href":"http:\/\/www.tangning.net\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/1159","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/www.tangning.net\/wordpress\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.tangning.net\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.tangning.net\/wordpress\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.tangning.net\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=1159"}],"version-history":[{"count":15,"href":"http:\/\/www.tangning.net\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/1159\/revisions"}],"predecessor-version":[{"id":1176,"href":"http:\/\/www.tangning.net\/wordpress\/index.php?rest_route=\/wp\/v2\/posts\/1159\/revisions\/1176"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/www.tangning.net\/wordpress\/index.php?rest_route=\/wp\/v2\/media\/1152"}],"wp:attachment":[{"href":"http:\/\/www.tangning.net\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=1159"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.tangning.net\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=1159"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.tangning.net\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=1159"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}