{"id":984,"date":"2017-09-25T00:18:08","date_gmt":"2017-09-24T16:18:08","guid":{"rendered":"http:\/\/c.tangning.net\/wordpress\/?post_type=al_product&p=984"},"modified":"2022-09-16T20:38:59","modified_gmt":"2022-09-16T12:38:59","slug":"ti2-lapp-%e6%a8%a1%e5%9d%97%e5%8c%96%e7%85%a7%e6%98%8e%e7%b3%bb%e7%bb%9f","status":"publish","type":"al_product","link":"http:\/\/www.tangning.net\/wordpress\/?product=ti2-lapp-%e6%a8%a1%e5%9d%97%e5%8c%96%e7%85%a7%e6%98%8e%e7%b3%bb%e7%bb%9f","title":{"rendered":"Ti2-LAPP \u6a21\u5757\u5316\u7167\u660e\u7cfb\u7edf"},"content":{"rendered":"

\"\"<\/p>\n

\n

 <\/p>\n

DMD\u6a21\u5757<\/h2>\n

 <\/p>\n

\u5b9e\u73b0\u540c\u65f6\u591a\u70b9\u5149\u6d3b\u5316<\/h2>\n

DMD\u6a21\u5757\u53ef\u5b9e\u73b0\u7528\u6237\u6307\u5b9a\u7684\u56fe\u6848\u548c\u4f4d\u7f6e\u7684\u5149\u6fc0\u6d3b\u548c\u5149\u7535\u8f6c\u6362\uff0c\u800c\u4f20\u7edf\u7684FRAP\u5355\u5143\u4ec5\u80fd\u591f\u5bf9\u5355\u4e2a\u624b\u52a8\u5b9a\u4f4d\u7684\u5149\u70b9\u8fdb\u884c\u5149\u6d3b\u5316\u3002 DMD\u7167\u660e\u5f62\u72b6\uff0c\u5c3a\u5bf8\uff0c\u4f4d\u7f6e\u548c\u6570\u91cf\u53ef\u4ee5\u4f7f\u7528NIS-Elements\u8f6f\u4ef6\u81ea\u7531\u5b9a\u5236\u3002\u8be5\u529f\u80fd\u5141\u8bb8\u7814\u7a76\u4eba\u5458\u5728\u5355\u4e2a\u7ec6\u80de\u6216\u591a\u4e2a\u7ec6\u80de\u4e2d\u5149\u5b66\u6807\u8bb0\u7ec6\u80de\u6216\u86cb\u767d\u8d28\u7fa4\u4f53\u7684\u5b50\u96c6\uff0c\u4ee5\u8ffd\u8e2a\u5176\u884c\u4e3a\u3002DMD\u6a21\u5757\u4e5f\u6700\u9002\u5408\u4e8e\u5149\u9057\u4f20\u5b66\u5b9e\u9a8c\uff0c\u5176\u4e2d\u9ad8\u5ea6\u5b9a\u5236\u7684ROI\u53ef\u7528\u4e8e\u5149\u5b66\u8bf1\u5bfc\u7ec6\u80de\u6216\u86cb\u767d\u8d28\u7fa4\u4f53\u6216\u4e9a\u7fa4\u7684\u529f\u80fd\u53d8\u5316\u3002DMD\u6a21\u5757\u53ef\u7528\u4e8e\u6fc0\u5149\u7167\u660e\u6216\u8f83\u5c11\u5149\u6bd2\u6027\u7684LED\u7167\u660e\u3002<\/p>\n

\"\"<\/p>\n

\"\"<\/p>\n

A mouse embryonic fibroblast co-expressing mCherry-tagged lamin A (red) and photo-activatable GFP-tagged lamin A was photo-converted (green) in the lower right region using the DMD module and 405 nm LED light. Time-lapse images were captured using the epi-fluorescence illuminator. By photoactivating a sub-population of the lamin proteins, one can observe their dynamics and subunit-exchange behavior.
\nImage courtesy of Drs. Takeshi Shimi and Bob Goldman, Northwestern University Medical School<\/p>\n

 <\/p>\n

\n

 <\/p>\n

H-TIRF\u6a21\u5757<\/h2>\n

 <\/p>\n

\u73b0\u5728\u53ef\u4ee5\u8fdb\u884c\u5168\u81ea\u52a8TIRF\u8c03\u6574\u548c\u89c2\u5bdf<\/h2>\n

\u6fc0\u5149\u5bf9\u4e8eTIRF\u89c2\u5bdf\u7684\u7406\u60f3\u5165\u5c04\u89d2\u548c\u7126\u70b9\u53d6\u51b3\u4e8e\u6837\u672c\u548c\u89c2\u5bdf\u6761\u4ef6\u3002\u8c03\u6574\u5165\u5c04\u89d2\u5ea6\u5e76\u805a\u7126\u4ee5\u5b9e\u73b0TIRF\u9700\u8981\u6280\u80fd\u548c\u7ecf\u9a8c\u3002H-TIRF\u6a21\u5757\u901a\u8fc7\u76d1\u6d4b\u53cd\u5c04\u5149\u675f\u81ea\u52a8\u8c03\u6574\u6fc0\u5149\u7684\u805a\u7126\u548c\u5165\u5c04\u89d2\uff0c\u7528\u4e8eTIRF\u89c2\u5bdf\u3002\u901a\u8fc7NIS-Elements\u8f6f\u4ef6\u4e2d\u7684\u81ea\u52a8\u5bf9\u51c6\u529f\u80fd\u8fdb\u884c\u81ea\u52a8\u6fc0\u5149\u805a\u7126\u8c03\u6574\u548c\u5165\u5c04\u89d2\u5ea6\u8c03\u6574\u3002\u53ef\u4ee5\u4fdd\u5b58\u548c\u518d\u73b0\u6e10\u901d\u573a\u7684\u5165\u5c04\u89d2\u548c\u7a7f\u900f\u6df1\u5ea6\uff0c\u7528\u4e8e\u540e\u7eed\u5b9e\u9a8c\uff0c\u4ee5\u786e\u4fdd\u4e00\u81f4\u7684\u6210\u50cf\u7ed3\u679c\u3002<\/p>\n

\"\"<\/p>\n

\"\"<\/p>\n

An in vitro preparation of fluorescently-labeled microtubules (tetramethylrhodamine and Alexa 647) and tubulin binding proteins (Alexa 488) was imaged in three different wavelengths using the H-TIRF illuminator and the gradation ND filter. Incident angles can be automatically adjusted for multiple wavelengths.
\nThe video of this image is in the \u201cSample Images\u201d page.
\nImage courtesy of Melissa Hendershott and Dr. Ron Vale, University of California, San Francisco<\/p>\n

\u6ca1\u6709\u7070\u5ea6\u7ea7ND\u6ee4\u955c\uff0cTIRF\u7167\u660e\u5728FOV\u4e2d\u663e\u793a\u9ad8\u65af\u5206\u5e03\uff0c\u4e2d\u5fc3\u662f\u6700\u4eae\u7684\u3002
\n\u4f7f\u7528\u7070\u5ea6\u7ea7ND\u6ee4\u955c\uff0c\u5b9e\u73b0\u4e86\u975e\u5e38\u5747\u5300\u7684TIRF\u7167\u660e\u3002<\/p>\n

\"\"<\/p>\n

An in vitro preparation of a bi-lipid membrane containing Alexa 488 (green)- and Alexa 561 (red)-tagged membrane-associated proteins was imaged using the H-TIRF illuminator and two different wavelengths (dual color TIRF). The proteins aggregate to form clusters that are visualized as circular structures (the top row).
\nThe lower row shows the 488 channels displayed using a rainbow look-up table where different intensities are represented as different colors.
\nThe H-TIRF illuminator can be used to automatically achieve optimal incident angles for the different wavelengths.
\nImage courtesy of Drs. Xiaolei Su and Ron Vale, University of California, San Francisco<\/p>\n

 <\/p>\n

\n

 <\/p>\n

N-STORM\u6a21\u5757<\/h2>\n

 <\/p>\n

\u5b9e\u73b0\u6bd4\u5e38\u89c4\u5149\u5b66\u663e\u5fae\u955c\u63d0\u9ad810\u500d\u7684\u5206\u8fa8\u7387<\/h2>\n

\u914d\u5907\u7167\u660e\u573a\uff081x\uff0c2x\uff0c4x\uff09\u7535\u52a8\u5f00\u5173\u4ee5\u53ca\u81ea\u52a8\u5bf9\u51c6\u529f\u80fd\uff0c\u8be5\u6a21\u5757\u53ef\u4e0eTi2-LAPP\u7cfb\u7edf\u8fdb\u884cN-STORM\u8d85\u5206\u8fa8\u7387\u6210\u50cf\u3002\u8fd9\u63d0\u4f9b\u4e86\u5927\u7ea620nm\u7684\u4ee4\u4eba\u96be\u4ee5\u7f6e\u4fe1\u7684\u56fe\u50cf\u5206\u8fa8\u7387\uff0c\u5176\u662f\u5e38\u89c4\u5149\u5b66\u663e\u5fae\u955c\u7684\u6781\u9650\u768410\u500d\u6216\u66f4\u5927\u3002\u5229\u7528STochastic\u5149\u5b66\u5206\u8fa8\u663e\u5fae\u955c\uff08STORM\uff09\uff0c\u53ef\u4ee5\u5728\u5206\u5b50\u6c34\u5e73\u4e0a\u6df1\u5165\u4e86\u89e3\u86cb\u767d\u8d28 – \u86cb\u767d\u8d28\u7684\u76f8\u4e92\u4f5c\u7528\u3002<\/p>\n

\"\"<\/p>\n

\n

 <\/p>\n

FRAP\u6a21\u5757<\/h2>\n

 <\/p>\n

\u7528\u4e8e\u5206\u6790\u7ec6\u80de\u5185-\u86cb\u767d\u8d28\u52a8\u529b\u5b66<\/h2>\n

\u4f7f\u7528\u8fd9\u79cdFRAP\u6a21\u5757\uff0c\u53ef\u4ee5\u8fdb\u884c\u5149\u6f02\u767d\u548c\u5149\u6fc0\u6d3b\/\u8f6c\u6362\u5b9e\u9a8c\u4ee5\u53ca\u4f7f\u7528\u9ad8\u5e27\u7387\uff0c\u9ad8\u7075\u654f\u5ea6\u7684\u76f8\u673a\u8fdb\u884c\u68c0\u6d4b\u3002\u8be5\u6a21\u5757\u53ef\u4ee5\u70b9\u4eae\u7ec6\u80de\u4e2d\u7684\u76ee\u6807\u4f4d\u7f6e\uff0c\u4e3a\u7814\u7a76\u7ec6\u80de\u5185\u86cb\u767d\u8d28\u52a8\u529b\u5b66\u800c\u4e0d\u4f7f\u7528\u70b9\u626b\u63cf\u5171\u7126\u663e\u5fae\u955c\u63d0\u4f9b\u4e86\u4f4e\u6210\u672c\u9ad8\u6548\u76ca\u7684\u65b9\u6cd5\u3002<\/p>\n

\"\"<\/p>\n

\"\"<\/p>\n

A mouse embryonic fibroblast expressing mCherry-lamin A was spot-photobleached in the upper right corner of the nucleus using the FRAP module to study the dynamics of lamin A molecules. Time-lapse images were acquired using the epi-fluorescence illuminator.
\nImage courtesy of Drs. Takeshi Shimi and Bob Goldman, Northwestern University Medical School<\/p>\n

 <\/p>\n

\n

 <\/p>\n

TIRF\u6a21\u5757<\/h2>\n

 <\/p>\n

\u7528\u4e8e\u89c2\u5bdf\u7ec6\u80de\u819c\u52a8\u529b\u5b66\u548c\u5355\u5206\u5b50<\/h2>\n

\u624b\u52a8TIRF\u6a21\u5757\u5305\u62ec\u4e00\u4e2a\u7070\u5ea6\u7ea7ND\u6ee4\u955c\uff08\u7c7b\u4f3c\u4e8eH-TIRF\u6a21\u5757\uff09\uff0c\u80fd\u591f\u5728\u6574\u4e2a\u89c6\u91ce\u5185\u5b9e\u73b0\u5747\u5300\u7684TIRF\u7167\u660e\u3002\u4f7f\u7528\u9ad8\u7075\u654f\u5ea6\u6444\u50cf\u673a\uff0c\u53ef\u4ee5\u4f7f\u7528\u8be5TIRF\u7167\u660e\u5668\u5728\u7ec6\u80de\u819c\u5185\u548c\u9644\u8fd1\u6210\u50cf\u5355\u5206\u5b50\u548c\u86cb\u767d\u8d28\u7684\u52a8\u529b\u5b66\u3002<\/p>\n

\"\"<\/p>\n

\n

 <\/p>\n

\u5927\u89c6\u91ce\u843d\u5c04\u8367\u5149\u6a21\u5757<\/h2>\n

 <\/p>\n

\u9002\u7528\u4e8e\u5e26\u6709\u5927\u578b\u4f20\u611f\u5668\u7684\u76f8\u673a\u7684\u8367\u5149\u6210\u50cf<\/h2>\n

\u7528\u4e8e\u5927\u578bFOV\u7684EPI FL\u6a21\u5757\u662fTi2-LAPP\u7cfb\u7edf\u7684\u57fa\u672c\u843d\u5c04\u8367\u5149\u7167\u660e\u5668\uff0c\u4e13\u4e3a\u4f7f\u7528Ti2\u5012\u7f6e\u663e\u5fae\u955c\u7684\u5927\u578bFOV\u6210\u50cf\u5e94\u7528\u800c\u8bbe\u8ba1\u3002 \u5373\u4f7f\u5b83\u5177\u6709\u7d27\u51d1\u7684\u8bbe\u8ba1\uff0c\u5b83\u5728\u843d\u5c04\u5149\u89c2\u5bdf\u671f\u95f4\u63d0\u4f9b\u65e0\u4e0e\u4f26\u6bd4\u7684\u5927\u768425mm\u89c6\u91ce\u3002\u5b83\u914d\u5907\u4e86\u77f3\u82f1\u590d\u773c\u955c\u7247\uff0c\u4ee5\u786e\u4fdd\u6574\u4e2a\u89c6\u91ce\u7684\u5747\u5300\u5f3a\u5ea6\uff0c\u5e76\u5728\u5e7f\u6cdb\u7684\u5149\u8c31\uff08\u5305\u62ec\u7d2b\u5916\u7ebf\uff09\u4e0b\u63d0\u4f9b\u9ad8\u900f\u5c04\u7387\u3002<\/p>\n

\"\"<\/p>\n

\n

 <\/p>\n

\u7075\u6d3b\u7ec4\u5408\u6a21\u5757<\/h2>\n

\"\"\u00a0\u00a0\u00a0\u00a0\"\"<\/p>\n

Ti2-LAPP\u7cfb\u7edf\u7684\u6a21\u5757\u5316\u548c\u7075\u6d3b\u914d\u7f6e\u529f\u80fd\u4e3a\u4e2a\u4eba\u7814\u7a76\u9700\u6c42\u63d0\u4f9b\u5b9a\u5236\u6210\u50cf\u89e3\u51b3\u65b9\u6848\u3002\u6a21\u5757\u4e5f\u53ef\u4ee5\u8f7b\u677e\u4ea4\u6362\u6216\u6dfb\u52a0\uff0c\u4ee5\u9002\u5e94\u4e0d\u65ad\u53d8\u5316\u7684\u5b9e\u9a8c\u9700\u6c42\uff0c\u8fd9\u662f\u5b9e\u9a8c\u5ba4\u7684\u91cd\u8981\u529f\u80fd\uff0c\u5177\u6709\u4e0d\u65ad\u53d1\u5c55\u7684\u7814\u7a76\u65b9\u5411\u548c\u591a\u7528\u6237\u6838\u5fc3\u8bbe\u65bd\u3002\u4f8b\u5982\uff0c\u901a\u8fc7\u5728\u5355TIRF\u914d\u7f6e\u4e2d\u6dfb\u52a0\u7b2c\u4e8c\u4e2aTIRF\u6a21\u5757\uff0c\u7528\u6237\u53ef\u4ee5\u8f7b\u677e\u6267\u884c\u5404\u5411\u5f02\u6027\u5b9e\u9a8c\u548c\u5feb\u901f\uff0c\u591a\u89d2\u5ea6TIRF\u5b9e\u9a8c\u3002\u6dfb\u52a0\u8bf8\u5982DMD\u6216FRAP\u6a21\u5757\u4e4b\u7c7b\u7684\u5149\u6fc0\u6d3b\/\u8f6c\u6362\u6a21\u5757\u53ef\u4ee5\u8ddf\u8e2a\u86cb\u767d\u8d28\u7fa4\u4f53\u7684\u4e9a\u5206\u652f\uff0c\u4ece\u800c\u63d0\u4f9b\u5bf9\u6210\u50cf\u6574\u4e2a\u7fa4\u4f53\u65f6\u7684\u865a\u5047\u4fe1\u606f\u7684\u86cb\u767d\u8d28\u884c\u4e3a\u7684\u89c1\u89e3\u3002<\/p>\n

\n

 <\/p>\n

\u53cc\u5c42\u914d\u7f6e\u529f\u80fd<\/h2>\n

\"\"\u00a0\u00a0\u00a0\u00a0\"\"<\/p>\n

\u5229\u7528\u5c3c\u5eb7Ti2\u7684\u5c42\u6b21\u7ed3\u6784\uff0c\u6a21\u5757\u53ef\u4ee5\u4f5c\u4e3a\u4e24\u5c42\u72ec\u7acb\u7684\u5c42\u53e0\uff0c\u6bcf\u5c42\u6709\u591a\u4e2a\u6a21\u5757\u3002\u4f7f\u7528\u53cc\u5c42\u914d\u7f6e\u53ef\u4e3a\u6bcf\u4e2a\u7167\u660e\u6a21\u5757\u5b9e\u73b0\u6700\u4f73\u7684\u8fc7\u6ee4\u5668\u914d\u7f6e\u3002\u4f8b\u5982\uff0c\u901a\u8fc7\u5c06H-TIRF\u6a21\u5757\u653e\u7f6e\u5728\u4e0b\u5c42\u4e2d\uff0c\u5e76\u4e14\u5728\u4e0a\u5c42\u4e2d\u8bbe\u7f6eDMD\u6a21\u5757\uff0c\u53ef\u4ee5\u5c06\u7279\u5b9a\u4e8eTIRF\u6210\u50cf\u548c\u5149\u6d3b\u5316\u7684\u5355\u72ec\u7684\u6ee4\u5149\u5757\u540c\u65f6\u7528\u4e8e\u5b83\u4eec\u5404\u81ea\u4f4d\u4e8e\u4e0b\u5c42\u548c\u4e0a\u5c42\u7684\u8f6c\u8f6e\u4e2d\u3002\u8be5\u914d\u7f6e\u53ef\u5b9e\u73b0\u6700\u4f73\u7684\u6ee4\u5149\u5757\u9009\u62e9\uff0c\u5e76\u63d0\u9ad8\u5b9e\u9a8c\u7cbe\u5ea6\uff0c\u540c\u65f6\u4fdd\u6301\u6700\u9ad8\u7684\u91c7\u96c6\u901f\u5ea6\u3002<\/p>\n\n\n\n
\"\"<\/th>\n\n

A Drosophila S2 cell expressing EOS-tubulin. The end of a single microtubule was photoconverted using the DMD module and 405 nm LED light. Time-lapse images in dual color TIRF were acquired using the H-TIRF illuminator. The addition of unconverted, green tubulin to the growing end of the photoconverted red microtubule, and shortening (and eventual disappearance) of the photoconverted segment demonstrate the dynamic instability property of microtubules. Arrowheads mark the growing and shrinking end of the photoconverted microtubule.
\nImage courtesy of Drs. Nico Stuurman and Ron Vale, University of California, San Francisco<\/p>\n<\/th>\n<\/tr>\n<\/thead>\n<\/table>\n","protected":false},"excerpt":{"rendered":"

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