{"id":846,"date":"2017-09-23T13:39:38","date_gmt":"2017-09-23T05:39:38","guid":{"rendered":"http:\/\/c.tangning.net\/wordpress\/?post_type=al_product&p=846"},"modified":"2020-02-29T15:50:28","modified_gmt":"2020-02-29T07:50:28","slug":"%e8%b6%85%e9%ab%98%e5%88%86%e8%be%a8%e7%8e%87%e6%98%be%e5%be%ae%e9%95%9c-n-sim-e","status":"publish","type":"al_product","link":"http:\/\/www.tangning.net\/wordpress\/?product=%e8%b6%85%e9%ab%98%e5%88%86%e8%be%a8%e7%8e%87%e6%98%be%e5%be%ae%e9%95%9c-n-sim-e","title":{"rendered":"\u8d85\u9ad8\u5206\u8fa8\u7387\u663e\u5fae\u955c N-SIM E"},"content":{"rendered":"

\"\"<\/p>\n

\n

 <\/p>\n

\u5c06\u4f20\u7edf\u5149\u5b66\u663e\u5fae\u955c\u7684\u5206\u8fa8\u7387\u63d0\u9ad8\u4e00\u500d<\/h2>\n

N-SIM E\u91c7\u7528\u5c3c\u5eb7\u521b\u65b0\u7684\u201c\u7ed3\u6784\u5316\u7167\u660e\u663e\u5fae\u955c\u201d\u6280\u672f\u3002 \u901a\u8fc7\u5c06\u8fd9\u79cd\u5f3a\u5927\u7684\u6280\u672f\u4e0e\u5c3c\u5eb7\u7740\u540d\u7684CFI Apochromat TIRF 100XC Oil\u76ee\u6807\uff08NA 1.49\uff09\u914d\u5bf9\uff0cN-SIM E\u51e0\u4e4e\u5c06\u4f20\u7edf\u5149\u5b66\u663e\u5fae\u955c\u7684\u7a7a\u95f4\u5206\u8fa8\u7387\uff08\u7ea6115 nm *\uff09\u63d0\u9ad8\u4e86\u4e00\u500d\uff0c\u5e76\u80fd\u591f\u7ec6\u5316\u7ec6\u80de\u5185\u7ed3\u6784\u548c\u4e92\u52a8\u529f\u80fd\u3002<\/p>\n

*\u8be5\u503c\u662f\u6d4b\u91cf\u57283D-SIM\u6a21\u5f0f\u4e0b\u7528488nm\u6fc0\u5149\u6fc0\u53d1\u7684100nm\u73e0\u7684FWHM\u3002 \u5728TIRF-SIM\u6a21\u5f0f\u4e0b\uff0c\u4f7f\u7528\u7528488nm\u6fc0\u5149\u6fc0\u53d1\u768440nm\u73e0\u7c92\u53ef\u5b9e\u73b086nm\u3002<\/p>\n

\"\"<\/p>\n

N-SIM image (3D-SIM)<\/p>\n

\"\"<\/p>\n

Conventional widefield image<\/p>\n

 <\/p>\n

Microtubules in B16 melanoma cell labeled with YFP
\nObjective: CFI Apochromat TIRF 100XC Oil (NA 1.49)
\nImage capturing speed: approximately 1.8 sec\/frame (movie)
\nReconstruction method: Slice
\nPhotographed with the cooperation of: Dr. Yasushi Okada, Laboratory for Cell Polarity Regulation, Quantitative Biology Center, RIKEN<\/p>\n

\"\"<\/p>\n

N-SIM image (3D-SIM)<\/p>\n

\"\"<\/p>\n

Conventional widefield image<\/p>\n

 <\/p>\n

Endoplasmic reticulum (ER) in living HeLa cell labeled with GFP
\nObjective: CFI Apochromat TIRF 100XC Oil (NA 1.49)
\nImage capturing speed: approximately 1.5 sec\/frame (movie)
\nReconstruction method: Slice
\nPhotographed with the cooperation of: Dr. Ikuo Wada, Institute of Biomedical Sciences, Fukushima Medical University School of Medicine<\/p>\n

\n

 <\/p>\n

\u5feb\u901f1\u79d2\/\u5e27\u65f6\u95f4\u5206\u8fa8\u7387\uff0c\u7528\u4e8e\u8d85\u5206\u8fa8\u7387\u6210\u50cf<\/h2>\n

N-SIM E\u4e3a\u7ed3\u6784\u5316\u7167\u660e\u6280\u672f\u63d0\u4f9b\u5feb\u901f\u6210\u50cf\u6027\u80fd\uff0c\u65f6\u95f4\u5206\u8fa8\u7387\u7ea6\u4e3a1\u79d2\/\u5e27\uff0c\u8fd9\u5bf9\u4e8e\u6d3b\u7ec6\u80de\u6210\u50cf\u662f\u975e\u5e38\u6709\u7528\u7684\u3002<\/p>\n

\n

 <\/p>\n

\u83b7\u53d6\u66f4\u5927\u7684\u89c6\u91ce<\/h2>\n

\u53ef\u4ee5\u83b7\u5f97\u5177\u670966\u03bcm\u6b63\u65b9\u5f62\u7684\u89c6\u91ce\u7684\u8d85\u5206\u8fa8\u7387\u56fe\u50cf\uff0c\u6bd4\u5e38\u89c4\u6210\u50cf\u533a\u57df\u5927\u56db\u500d\u3002 \u6269\u5927\u7684\u6210\u50cf\u533a\u57df\u5927\u5927\u63d0\u9ad8\u4e86\u4ece\u66f4\u5927\u89c6\u91ce\u53d7\u76ca\u7684\u5e94\u7528\/\u6837\u672c\u7684\u541e\u5410\u91cf\uff0c\u4f8b\u5982\u795e\u7ecf\u5143\uff0c\u51cf\u5c11\u83b7\u5f97\u5fc5\u8981\u6570\u636e\u6240\u9700\u7684\u65f6\u95f4\u548c\u7cbe\u529b\u3002<\/p>\n

\"\"<\/p>\n

Reconstructed image size: 1024 x 1024 pixels (33 \u03bcm x 33 \u03bcm with a 100X objective)<\/p>\n

\"\"<\/p>\n

Reconstructed image size: 2048 x 2048 pixels (66 \u03bcm x 66 \u03bcm with a 100X objective)<\/p>\n

 <\/p>\n

Growth cone of NG108 cell labelled with TRITC-phalloidin (F-actin, orange) and Alexa Fluor\u00ae 488 (microtubules, green)
\nSample courtesy of: Dr. Shizuha Ishiyama and Dr. Kaoru Katoh, The National Institute of Advanced Industrial Science and Technology (AIST)<\/p>\n

\n

 <\/p>\n

\u5177\u67093D-SIM\u6a21\u5f0f\u7684\u8f74\u5411\u8d85\u9ad8\u5206\u8fa8\u7387\u6210\u50cf<\/h2>\n

\u6709\u4e24\u79cd\u91cd\u5efa\u65b9\u6cd5\u53ef\u7528\u3002 \u5207\u7247\u91cd\u5efa\u5141\u8bb8\u5728\u6d3b\u7ec6\u80de\u6837\u672c\u4e2d\u4ee5300nm\u5206\u8fa8\u7387\u8fdb\u884c\u5149\u5b66\u5207\u7247\u7684\u8f74\u5411\u8d85\u5206\u8fa8\u7387\u6210\u50cf\u3002 \u53ef\u9009\u7684\u5806\u53e0\u91cd\u5efa\u53ef\u4ee5\u5bf9\u5207\u7247\u91cd\u5efa\u7684\u5bf9\u6bd4\u5ea6\u8f83\u9ad8\u7684\u56fe\u50cf\u8fdb\u884c\u6210\u50cf\u3002
\n\"\"<\/p>\n

N-SIM image (3D-SIM)<\/p>\n

\"\"<\/p>\n

Conventional widefield image<\/p>\n

 <\/p>\n

Bacillus subtilis bacterium stained with membrane dye Nile Red (red), and expressing the cell division protein DivIVA fused to GFP (green).
\nThe superior resolution of N-SIM system allows for accurate localization of the protein during division.
\nReconstruction method: Slice
\nPhotos courtesy of: Drs. Henrik Strahl and Leendert Hamoen, Centre for Bacterial Cell Biology, Newcastle University<\/p>\n

\"\"<\/p>\n

3D-SIM (Volume view)<\/p>\n

\"\"<\/p>\n

3D-SIM (Maximum projection)<\/p>\n

 <\/p>\n

Mouse keratinocyte labeled with an antibody against keratin intermediate filaments and stained with an Alexa Fluor\u00ae 488 conjugated second antibody.
\nReconstruction method: Stack
\nPhotos courtesy of: Dr. Reinhard Windoffer, RWTH Aachen University<\/p>\n

\n

 <\/p>\n

\u7b80\u6613\u7684\u6210\u50cf\u65b9\u6cd5\u5207\u6362<\/h2>\n

\u53ef\u4ee5\u4f7f\u7528\u5171\u7126\u663e\u5fae\u955c\u7cfb\u7edf\uff08\u5982A1+ \u6216 C2+\uff09\u540c\u65f6\u914d\u7f6eN-SIM E\uff0c\u5e76\u4e14\u53ef\u4ee5\u5728\u8d85\u5206\u8fa8\u7387\u6210\u50cf\u548c\u5171\u805a\u7126\u6210\u50cf\u4e4b\u95f4\u8f7b\u677e\u5207\u6362\u6210\u50cf\u65b9\u6cd5\u3002
\n\u53ef\u4ee5\u5728\u5171\u7126\u56fe\u50cf\u4e2d\u6307\u5b9aSIM\u56fe\u50cf\u7684\u671f\u671b\u4f4d\u7f6e\u5e76\u4ee5\u8d85\u5206\u8fa8\u7387\u83b7\u53d6\u3002<\/p>\n

\"\"<\/p>\n

\n

 <\/p>\n

3\u8272\u591a\u6fc0\u5149\u8d85\u5206\u8fa8\u80fd\u529b<\/h2>\n

\u7528\u4e8eN-SIM E\u7684\u7d27\u51d1\u578bLU-N3-SIM\u6fc0\u5149\u5355\u5143\u5b89\u88c5\u4e86\u4e09\u79cd\u6700\u5e38\u7528\u7684\u6ce2\u957f\u6fc0\u5149\u5668\uff08488\/561\/640\uff09\uff0c\u53ef\u5b9e\u73b0\u591a\u79cd\u989c\u8272\u7684\u8d85\u9ad8\u5206\u8fa8\u7387\u6210\u50cf\u3002 \u5b83\u80fd\u591f\u5728\u5206\u5b50\u6c34\u5e73\u4e0a\u7814\u7a76\u591a\u79cd\u611f\u5174\u8da3\u7684\u86cb\u767d\u8d28\u7684\u52a8\u6001\u76f8\u4e92\u4f5c\u7528\u3002
\n\"\"<\/p>\n

\n

 <\/p>\n

\u8d85\u5206\u8fa8\u7387\u663e\u5fae\u955c\u7684\u7269\u955c<\/h2>\n

 <\/p>\n

\u7845\u80f6\u6d78\u6e0d\u7269\u955c<\/h2>\n

\u7845\u80f6\u6d78\u6e0d\u7269\u955c\u91c7\u7528\u9ad8\u7c98\u5ea6\u7845\u6cb9\u6d78\u6e0d\u6db2\u4f53\uff0c\u5176\u6298\u5c04\u7387\u4e0e\u6d3b\u7ec6\u80de\u7684\u6298\u5c04\u7387\u5bc6\u5207\u76f8\u5173\u3002 \u5b83\u5141\u8bb8\u5728\u957f\u671f\uff0c\u5ef6\u65f6\u6210\u50cf\u671f\u95f4\u6355\u83b7\u9ad8\u5206\u8fa8\u7387\uff0c\u591a\u82723D\u56fe\u50cf\u76f4\u5230\u7ec6\u80de\u7684\u9876\u7aef\u3002 \u901a\u8fc7\u5bbd\u6ce2\u957f\u8303\u56f4\u786e\u4fdd\u5353\u8d8a\u7684\u8272\u5dee\u6821\u6b63\u548c\u9ad8\u900f\u5c04\u7387\u3002
\n\"\"<\/p>\n

CFI SR HP Plan Apochromat Lambda S 100XC Sil<\/p>\n

\n

 <\/p>\n

\u6d78\u6e0d\u7269\u955c<\/h2>\n

\u4f7f\u7528\u6ce2\u524d\u50cf\u5dee\u6d4b\u91cf\u7684\u955c\u5934\u77eb\u6b63\u548c\u63a2\u6d4b\u4e3a\u8d85\u5206\u8fa8\u7387\u663e\u5fae\u955c\u63d0\u4f9b\u4e86\u8d85\u7ea7\u5149\u5b66\u6027\u80fd\u7684SR\u955c\u5934\uff0c\u5c06\u4e0d\u5bf9\u79f0\u50cf\u5dee\u7684\u51fa\u73b0\u964d\u81f3\u6700\u4f4e\u6c34\u5e73\u3002
\nSR HP\u7269\u955c\u63d0\u4f9b\u9ad8\u8010\u7528\u6027\uff0c\u53ef\u5b9e\u73b0\u9ad8\u529f\u7387\u6fc0\u5149\u6fc0\u53d1\u548c\u6700\u5c0f\u8f74\u5411\u8272\u5dee\uff0c\u5e76\u4e14\u5728\u8d85\u5206\u8fa8\u7387\u6210\u50cf\u4e2d\u8868\u73b0\u51fa\u4f18\u5f02\u7684\u6027\u80fd\uff0c\u65e0\u9700\u5728N-SIM\u548cN-STORM\u7cfb\u7edf\u4e4b\u95f4\u5207\u6362\u7269\u955c\u3002
\n\"\"    \"\"<\/p>\n

Left:CFI SR HP Apochromat TIRF 100XC Oil; Right:CFI SR Plan Apochromat IR 60XC WI<\/p>\n

\n

 <\/p>\n

\u5e72\u955c\u5934<\/h2>\n

\u5e72\u955c\u5934\u73b0\u5728\u4e0eN-SIM\u4ee5\u53ca\u5171\u7126\u663e\u5fae\u955c\u517c\u5bb9\uff0c\u56e0\u6b64\u65e0\u9700\u5207\u6362\u955c\u5934\u5c31\u53ef\u4ee5\u4f7f\u7528\u5171\u805a\u7126\u6210\u50cf\u548c\u8d85\u5206\u8fa8\u7387\u6210\u50cf\u3002 \u4f4e\u500d\u7387\u548c\u5bbd\u89c6\u573a\u5e72\u900f\u955c\u53ef\u4ee5\u5728\u6837\u54c1\u7ec4\u7ec7\u7684\u5468\u56f4\u8fdb\u884c\u9ad8\u5206\u8fa8\u7387\u89c2\u5bdf\u3002
\n*\u5e72\u76ee\u6807\u652f\u63012D-SIM\u548c3D-SIM\uff08\u5207\u7247\u91cd\u5efa\uff09
\n\"\"    \"\"<\/p>\n

Left:CFI Plan Apochromat Lambda 60XC; Right:CFI Plan Apochromat Lambda 40XC<\/p>\n

\"\"<\/p>\n","protected":false},"excerpt":{"rendered":"

\u4e2a\u4eba\u8d85\u5206\u8fa8\u7387\u663e\u5fae\u955c\uff0c\u63d0\u4f9b\u4e0eN-SIM\u76f8\u540c\u7684\u9ad8\u5206\u8fa8\u7387<\/h2>\n

N-SIM E\u662f\u4e00\u79cd\u6d41\u7ebf\u578b\uff0c\u7ecf\u6d4e\u5b9e\u60e0\u7684\u8d85\u5206\u8fa8\u7387\u7cfb\u7edf\uff0c\u53ef\u63d0\u4f9b\u5e38\u89c4\u5149\u5b66\u663e\u5fae\u955c\u5206\u8fa8\u7387\u7684\u4e24\u500d\u3002\u7ed3\u5408N-SIM E\u548c\u5171\u805a\u7126\u663e\u5fae\u955c\uff0c\u60a8\u53ef\u4ee5\u7075\u6d3b\u5730\u9009\u62e9\u5171\u7126\u56fe\u50cf\u4e2d\u7684\u4f4d\u7f6e\uff0c\u5e76\u53ef\u8f7b\u677e\u5207\u6362\u5230\u8d85\u5206\u8fa8\u7387\u67e5\u770b\uff0c\u4ece\u800c\u83b7\u5f97\u66f4\u591a\u7ec6\u8282\u3002 \u8fdb\u5165\u7eb3\u7c73\u9006\u8f6c\u5f00\u521b\u4e86\u751f\u547d\u79d1\u5b66\u8fd9\u4e00\u65b0\u524d\u6cbf\u7684\u53ef\u80fd\u6027\u3002<\/p>\n","protected":false},"featured_media":847,"template":"","al_product-cat":[20],"class_list":["post-846","al_product","type-al_product","status-publish","has-post-thumbnail","hentry","al_product-cat-super-resolution"],"_links":{"self":[{"href":"http:\/\/www.tangning.net\/wordpress\/index.php?rest_route=\/wp\/v2\/al_product\/846","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/www.tangning.net\/wordpress\/index.php?rest_route=\/wp\/v2\/al_product"}],"about":[{"href":"http:\/\/www.tangning.net\/wordpress\/index.php?rest_route=\/wp\/v2\/types\/al_product"}],"wp:featuredmedia":[{"embeddable":true,"href":"http:\/\/www.tangning.net\/wordpress\/index.php?rest_route=\/wp\/v2\/media\/847"}],"wp:attachment":[{"href":"http:\/\/www.tangning.net\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=846"}],"wp:term":[{"taxonomy":"al_product-cat","embeddable":true,"href":"http:\/\/www.tangning.net\/wordpress\/index.php?rest_route=%2Fwp%2Fv2%2Fal_product-cat&post=846"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}