{"id":818,"date":"2017-09-23T10:59:05","date_gmt":"2017-09-23T02:59:05","guid":{"rendered":"http:\/\/c.tangning.net\/wordpress\/?post_type=al_product&p=818"},"modified":"2017-09-23T14:02:30","modified_gmt":"2017-09-23T06:02:30","slug":"%e8%b6%85%e9%ab%98%e5%88%86%e8%be%a8%e7%8e%87%e6%98%be%e5%be%ae%e9%95%9c-n-sim","status":"publish","type":"al_product","link":"http:\/\/www.tangning.net\/wordpress\/?product=%e8%b6%85%e9%ab%98%e5%88%86%e8%be%a8%e7%8e%87%e6%98%be%e5%be%ae%e9%95%9c-n-sim","title":{"rendered":"\u8d85\u9ad8\u5206\u8fa8\u7387\u663e\u5fae\u955c N-SIM"},"content":{"rendered":"

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\u6d3b\u7ec6\u80de\u6210\u50cf\u662f\u4f20\u7edf\u5149\u5b66\u663e\u5fae\u955c\u5206\u8fa8\u7387\u7684\u4e24\u500d<\/h2>\n

N-SIM\u4f7f\u7528\u5c3c\u5eb7\u521b\u65b0\u7684\u201c\u7ed3\u6784\u5316\u7167\u660e\u663e\u5fae\u955c\u201d\u6280\u672f\u3002\u901a\u8fc7\u4e0e\u5c3c\u5eb7\u8457\u540d\u7684CFI Apochromat TIRF 100XC Oil\u7269\u955c\uff08NA 1.49\uff09\u914d\u5bf9\uff0c\u8fd9\u79cd\u5f3a\u5927\u7684\u6280\u672f\u4e0eN-SIM\u51e0\u4e4e\u76f8\u5f53\u4e8e\u4f20\u7edf\u5149\u5b66\u663e\u5fae\u955c\u7684\u7a7a\u95f4\u5206\u8fa8\u7387\u7684\u4e24\u500d\uff08\u7ea6115nm *\uff09\uff0c\u5e76\u80fd\u591f\u7ec6\u5316\u7ec6\u80de\u5185\u7ed3\u6784\u548c\u4e92\u52a8\u529f\u80fd\u3002<\/p>\n

*\u8be5\u503c\u662f\u6d4b\u91cf\u57283D-SIM\u6a21\u5f0f\u4e0b\u7528488nm\u6fc0\u5149\u6fc0\u53d1\u7684100nm\u73e0\u7684FWHM\u3002 \u5728TIRF-SIM\u6a21\u5f0f\u4e0b\uff0c\u4f7f\u7528\u7528488nm\u6fc0\u5149\u6fc0\u53d1\u768440nm\u73e0\u7c92\u53ef\u5b9e\u73b086nm\u3002<\/p>\n

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Macrophages (J774 cells expressing mVenus-SNAP23) phagocytosing opsonized beads that were incubated with Alexa555 labeled secondary antibodies after fixation.
\nThe beads without red signals are in phagosomes containing mVenus-SNAP23.
\nPhotographed with the cooperation of: Drs. Chie Sakurai, Kiyotaka Hatsuzawa and Ikuo Wada, Fukushima Medical University School of Medicine.<\/p>\n

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N-SIM image (3D-SIM)<\/p>\n

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Conventional widefield image<\/p>\n

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Microtubules in B16 melanoma cell labeled with YFP
\nObjective: CFI Apochromat TIRF 100XC Oil (NA 1.49)
\nImage capturing speed: approximately 1.8 sec\/frame (movie)
\nReconstruction method: Slice
\nPhotographed with the cooperation of: Dr. Yasushi Okada, Laboratory for Cell Polarity Regulation, Quantitative Biology Center, RIKEN<\/p>\n

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N-SIM image (3D-SIM)<\/p>\n

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Conventional widefield image<\/p>\n

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Endoplasmic reticulum (ER) in living HeLa cell labeled with GFP
\nObjective: CFI Apochromat TIRF 100XC Oil (NA 1.49)
\nImage capturing speed: approximately 1.5 sec\/frame (movie)
\nReconstruction method: Slice
\nPhotographed with the cooperation of: Dr. Ikuo Wada, Institute of Biomedical Sciences, Fukushima Medical University School of Medicine<\/p>\n

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\u65f6\u95f4\u5206\u8fa8\u7387\u4e3a0.6\u79d2\/\u5e27\u60ca\u4eba\u7684\u8d85\u5206\u8fa8\u7387\u663e\u5fae\u955c<\/h2>\n

N-SIM\u4e3a\u7ed3\u6784\u5316\u7167\u660e\u6280\u672f\u63d0\u4f9b\u8d85\u5feb\u901f\u6210\u50cf\u529f\u80fd\uff0c\u65f6\u95f4\u5206\u8fa8\u7387\u9ad8\u8fbe0.6\u79d2\/\u5e27\uff0c\u5bf9\u4e8e\u6d3b\u7ec6\u80de\u6210\u50cf\uff08\u4f7f\u7528TIRF-SIM \/ 2D-SIM\u6a21\u5f0f;3D-SIM\u6210\u50cf\u9ad8\u8fbe\u7ea61 \u79d2\/\u5e27\uff08\u5207\u7247\u91cd\u5efa\uff09\uff09\u3002<\/p>\n

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Dynamics of mitochondria stained with Mito-Tracker red
\nCristae in mitochondria are visualized and the dynamics of mitochondria can be observed.
\nMode: 3D-SIM (slice reconstruction)
\nObjective: CFI Apochromat TIRF 100XC Oil (NA 1.49)
\nImage capturing interval: approximately 1 sec. (movie)<\/p>\n

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\u83b7\u53d6\u66f4\u5927\u7684\u89c6\u91ce<\/h2>\n

\u53ef\u4ee5\u83b7\u5f97\u5177\u670966\u03bcm\u6b63\u65b9\u5f62\u7684\u89c6\u91ce\u7684\u8d85\u5206\u8fa8\u7387\u56fe\u50cf\uff0c\u6bd4\u5e38\u89c4\u6210\u50cf\u533a\u57df\u5927\u56db\u500d\u3002 \u6269\u5927\u7684\u6210\u50cf\u533a\u57df\u5927\u5927\u63d0\u9ad8\u4e86\u4ece\u66f4\u5927\u89c6\u91ce\u53d7\u76ca\u7684\u5e94\u7528\/\u6837\u672c\u7684\u541e\u5410\u91cf\uff0c\u4f8b\u5982\u795e\u7ecf\u5143\uff0c\u51cf\u5c11\u83b7\u5f97\u5fc5\u8981\u6570\u636e\u6240\u9700\u7684\u65f6\u95f4\u548c\u7cbe\u529b\u3002<\/p>\n

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Reconstructed image size: 1024 x 1024 pixels (33 \u03bcm x 33 \u03bcm with a 100X objective)<\/p>\n

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Reconstructed image size: 2048 x 2048 pixels (66 \u03bcm x 66 \u03bcm with a 100X objective)<\/p>\n

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Growth cone of NG108 cell labelled with TRITC-phalloidin (F-actin, orange) and Alexa Fluor\u00ae 488 (microtubules, green)
\nSample courtesy of: Dr. Shizuha Ishiyama and Dr. Kaoru Katoh, The National Institute of Advanced Industrial Science and Technology (AIST)<\/p>\n

\n

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\u591a\u79cd\u89c2\u5bdf\u6a21\u5f0f<\/h2>\n

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TIRF-SIM \/ 2D-SIM\u6a21\u5f0f<\/h2>\n

\u8be5\u6a21\u5f0f\u4ee5\u60ca\u4eba\u7684\u5bf9\u6bd4\u5ea6\u9ad8\u901f\u6355\u83b7\u8d85\u5206\u8fa8\u73872D\u56fe\u50cf\u3002\u4e0e\u5e38\u89c4TIRF\u663e\u5fae\u955c\u76f8\u6bd4\uff0cTIRF-SIM\u6a21\u5f0f\u5229\u7528\u5168\u5185\u53cd\u5c04\u8367\u5149\u89c2\u5bdf\u7684\u4e24\u500d\u5206\u8fa8\u7387\uff0c\u6709\u52a9\u4e8e\u66f4\u597d\u5730\u4e86\u89e3\u7ec6\u80de\u8868\u9762\u4e0a\u7684\u5206\u5b50\u76f8\u4e92\u4f5c\u7528\u3002<\/p>\n

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TIRF-SIM image<\/p>\n

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Conventional TIRF image<\/p>\n

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Plasma membrane of B16 melanoma cell labeled with YFP
\nObjective: CFI Apochromat TIRF 100XC Oil (NA 1.49)
\nPhotographed with the cooperation of: Dr. Yasushi Okada, Laboratory for Cell Polarity Regulation, Quantitative Biology Center, RIKEN<\/p>\n

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3D-SIM\u6a21\u5f0f<\/h2>\n

3D-SIM\u7167\u660e\u6280\u672f\u5c06\u8f74\u5411\u5206\u8fa8\u7387\u63d0\u9ad8\u5230269 nm\uff0c\u5177\u6709\u6837\u54c1\u5149\u5b66\u5207\u7247\u7684\u80fd\u529b\uff0c\u80fd\u591f\u5728\u66f4\u9ad8\u7684\u7a7a\u95f4\u5206\u8fa8\u7387\u4e0b\u53ef\u89c6\u5316\u66f4\u7ec6\u81f4\u7684\u5355\u5143\u683c\u7ed3\u6784\u3002<\/p>\n

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Luminal surface of the organ of Corti at postnatal day 1. (Mouse)
\nGreen: F-actin, red: acetylated-tubulin
\nPhotographed with the cooperation of: Drs. Kanoko Kominami, Hideru Togashi, and Yoshimi Takai, Division of Molecular and Cellular Biology, Kobe University Graduate School of Medicine\/Faculty of Medicine<\/p>\n

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N-SIM image (3D-SIM)<\/p>\n

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Conventional widefield image<\/p>\n

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Bacillus subtilis bacterium stained with membrane dye Nile Red (red), and expressing the cell division protein DivIVA fused to GFP (green).
\nThe superior resolution of N-SIM system allows for accurate localization of the protein during division.
\nReconstruction method: Slice
\nPhotos courtesy of: Drs. Henrik Strahl and Leendert Hamoen, Centre for Bacterial Cell Biology, Newcastle University<\/p>\n

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3D-SIM (Volume view)<\/p>\n

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3D-SIM (Maximum projection)<\/p>\n

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Mouse keratinocyte labeled with an antibody against keratin intermediate filaments and stained with an Alexa Fluor\u00ae 488 conjugated second antibody.
\nReconstruction method: Stack
\nPhotos courtesy of: Dr. Reinhard Windoffer, RWTH Aachen University<\/p>\n

\n

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\u7b80\u6613\u7684\u6210\u50cf\u65b9\u6cd5\u5207\u6362<\/h2>\n

\u53ef\u4ee5\u4f7f\u7528\u5171\u7126\u663e\u5fae\u955c\u7cfb\u7edf\uff08\u5982A1 +\uff09\u540c\u65f6\u914d\u7f6eN-SIM\uff0c\u5e76\u4e14\u53ef\u4ee5\u5728\u8d85\u5206\u8fa8\u7387\u6210\u50cf\u548c\u5171\u805a\u7126\u6210\u50cf\u4e4b\u95f4\u8f7b\u677e\u5207\u6362\u6210\u50cf\u65b9\u6cd5\u3002
\n\u53ef\u4ee5\u5728\u5171\u7126\u56fe\u50cf\u4e2d\u6307\u5b9aSIM\u56fe\u50cf\u7684\u671f\u671b\u4f4d\u7f6e\u5e76\u4ee5\u8d85\u5206\u8fa8\u7387\u83b7\u53d6\u3002<\/p>\n

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5\u6fc0\u5149\u591a\u8272\u8d85\u5206\u8fa8\u6210\u50cf<\/h2>\n

\u5c3c\u5eb7N-SIM\u7cfb\u7edf\u5141\u8bb8\u591a\u8fbe5\u6fc0\u5149\u5668\u7684\u591a\u901a\u9053\u6210\u50cf\uff0c\u4f7f\u7814\u7a76\u5206\u5b50\u6c34\u5e73\u4e0a\u611f\u5174\u8da3\u7684\u591a\u4e2a\u86cb\u767d\u8d28\u7684\u52a8\u6001\u76f8\u4e92\u4f5c\u7528\u3002
\n\"\"<\/p>\n

Human U2OS cell during mitosis metaphase
\nThe cell is labeled green (kinetochore protein CENP-B), red (alpha-tubulin) and blue (DNA).
\nPhoto courtesy of: Dr. Alexey Khodjakov, Wadsworth Center, Albany NY<\/p>\n

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\u53cc\u6ce2\u957f\u540c\u6b65\u8d85\u5206\u8fa8\u7387\u6210\u50cf<\/h2>\n

\u901a\u8fc7\u4f7f\u7528\u53ef\u9009\u7684\u53cc\u6444\u50cf\u5934\u6210\u50cf\u9002\u914d\u5668*\u5c06\u4e24\u4e2aEMCCD\/sCMOS\u76f8\u673a\u8fde\u63a5\u5230\u663e\u5fae\u955c\uff0c\u53ef\u4ee5\u540c\u65f6\u8fdb\u884c488 nm\u548c561 nm\u6fc0\u53d1\u7684\u53cc\u6ce2\u957f\u8d85\u5206\u8fa8\u7387\u6210\u50cf\u3002<\/p>\n

*\u5b89\u9053\u5c14\u79d1\u6280\u6709\u9650\u516c\u53f8\u4ea7\u54c1<\/p>\n

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\u4e24\u53f0\u76f8\u673a\u6210\u50cf\u9002\u914d\u5668\uff08\u9002\u7528\u4e8eN-SIM\uff09
\n*\u5b9e\u9645\u4ea7\u54c1\u7684\u8bbe\u8ba1\u53ef\u80fd\u7565\u6709\u4e0d\u540c\u3002<\/p>\n

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