{"id":1097,"date":"2020-02-29T08:58:20","date_gmt":"2020-02-29T00:58:20","guid":{"rendered":"http:\/\/www.tangning.net\/wordpress\/?product=%e8%b6%85%e9%ab%98%e5%88%86%e8%be%a8%e7%8e%87%e6%98%be%e5%be%ae%e9%95%9c-n-sim-2"},"modified":"2020-02-29T14:56:53","modified_gmt":"2020-02-29T06:56:53","slug":"%e8%b6%85%e9%ab%98%e5%88%86%e8%be%a8%e7%8e%87%e6%98%be%e5%be%ae%e9%95%9c-n-sim-2","status":"publish","type":"al_product","link":"http:\/\/www.tangning.net\/wordpress\/?product=%e8%b6%85%e9%ab%98%e5%88%86%e8%be%a8%e7%8e%87%e6%98%be%e5%be%ae%e9%95%9c-n-sim-2","title":{"rendered":"N-SIM S \u5feb\u901f\u8d85\u9ad8\u5206\u8fa8\u7387\u663e\u5fae\u955c\u7cfb\u7edf"},"content":{"rendered":"<p><div style=\"width: 544px;\" class=\"wp-video\"><!--[if lt IE 9]><script>document.createElement('video');<\/script><![endif]-->\n<video class=\"wp-video-shortcode\" id=\"video-1097-1\" width=\"544\" height=\"272\" preload=\"metadata\" controls=\"controls\"><source type=\"video\/mp4\" src=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/vlc-output.mp4?_=1\" \/><a href=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/vlc-output.mp4\">http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/vlc-output.mp4<\/a><\/video><\/div><br \/>\n&nbsp;<\/p>\n<h2 class=\"c-heading-b c-accordion-local__section__title__heading\" style=\"font-size: 25px; color: #222222; background-color: #eeeeee;\">15fps\u7684\u9ad8\u901f\u8d85\u9ad8\u5206\u8fa8\u7387\u6210\u50cf<\/h2>\n<p>&nbsp;<br \/>\n\u5c3c\u5eb7\u7684\u65b0\u578b\u9ad8\u901f\u7ed3\u6784\u7167\u660e\u7cfb\u7edf\u91c7\u7528\u4e86\u65b0\u9896\u7684\u56fe\u50cf\u8c03\u5236\u6280\u672f\uff0c\u53ef\u4ee5\u5feb\u901f\uff0c\u7cbe\u786e\u5730\u5207\u6362\u7167\u660e\u56fe\u6848\u3002N-SIM S\u5b9e\u73b0\u4e86\u4ee4\u4eba\u96be\u4ee5\u7f6e\u4fe1\u7684\u91c7\u96c6\u901f\u5ea6\uff08\u9ad8\u8fbe15fps*\uff09\uff0c\u53ef\u5bf9\u6d3b\u7ec6\u80de\u548c\u7ec6\u80de\u5185\u52a8\u529b\u5b66\u8fdb\u884c\u8d85\u5206\u8fa8\u7387\u5ef6\u65f6\u6210\u50cf\u3002<\/p>\n<p style=\"font-size: 11px;\">*2D-SIM\u6a21\u5f0f\uff0c512 x 512\u50cf\u7d20\uff0c2\u6beb\u79d2\u66dd\u5149\u65f6\u95f4<\/p>\n<p>&nbsp;<br \/>\n<div style=\"width: 993px;\" class=\"wp-video\"><video class=\"wp-video-shortcode\" id=\"video-1097-2\" width=\"993\" height=\"344\" preload=\"metadata\" controls=\"controls\"><source type=\"video\/mp4\" src=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/20200228080801-871.mp4?_=2\" \/><a href=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/20200228080801-871.mp4\">http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/20200228080801-871.mp4<\/a><\/video><\/div><\/p>\n<p style=\"font-size: 9px;\">Endosomes of a COS7 cell labeled with YFP. Rapid movement of endosomes is captured at high resolution. This video shows a comparison with a widefield image. \u2028Image acquisition speed: 6 fps \u2028Imaging mode: 3D-SIM \u2028Image courtesy of: Yasushi Okada, M.D., Ph.D., Department of Physics, Graduate School of Science, The University of Tokyo<\/p>\n<p>&nbsp;<br \/>\n<div style=\"width: 816px;\" class=\"wp-video\"><video class=\"wp-video-shortcode\" id=\"video-1097-3\" width=\"816\" height=\"272\" preload=\"metadata\" controls=\"controls\"><source type=\"video\/mp4\" src=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/vlc-output1.mp4?_=3\" \/><a href=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/vlc-output1.mp4\">http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/vlc-output1.mp4<\/a><\/video><\/div><\/p>\n<p style=\"font-size: 9px;\">Growth cone of NG108 cell labeled with GFP-Lifeact for F-actin. Formation of an actin mesh is captured at high-speed. This video shows a comparison with a widefield image.<br \/>\nImage acquisition speed: 10 fps<br \/>\nImaging mode: TIRF-SIM<br \/>\nImage courtesy of: Drs. Minami Tanaka and Kaoru Katoh, The National Institute of Advanced Industrial Science and Technology (AIST)<\/p>\n<p>&nbsp;<br \/>\n<div style=\"width: 993px;\" class=\"wp-video\"><video class=\"wp-video-shortcode\" id=\"video-1097-4\" width=\"993\" height=\"333\" preload=\"metadata\" controls=\"controls\"><source type=\"video\/mp4\" src=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/20200228083315-877.mp4?_=4\" \/><a href=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/20200228083315-877.mp4\">http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/20200228083315-877.mp4<\/a><\/video><\/div><\/p>\n<p style=\"font-size: 9px;\">Histone H2B-GFP expressing HeLa cell. Visualization of fine movements of chromatin domains in different locations. This video shows a comparison with a widefield image.<br \/>\nImage acquisition speed: 3.9 fps<br \/>\nImaging mode: 3D-SIM<br \/>\nImage courtesy of: Yuko Sato, Ph.D. and Hiroshi Kimura, Ph.D., Cell Biology Center, Institute of Innovative Research, Tokyo Institute of Technology<\/p>\n<p>&nbsp;<br \/>\n<div style=\"width: 993px;\" class=\"wp-video\"><video class=\"wp-video-shortcode\" id=\"video-1097-5\" width=\"993\" height=\"331\" preload=\"metadata\" controls=\"controls\"><source type=\"video\/mp4\" src=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/20200228083532-827.mp4?_=5\" \/><a href=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/20200228083532-827.mp4\">http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/20200228083532-827.mp4<\/a><\/video><\/div><\/p>\n<p style=\"font-size: 9px;\">ER of COS7 cell labelled with SGFP2-sec61b. Fine movements of endoplasmic reticula can be captured and visualized with reconstruction. This video shows a comparison with a widefield image.<br \/>\nImage acquisition speed: 3.9 fps<br \/>\nImaging mode: 3D-SIM<br \/>\nImage courtesy of: Ikuo Wada, Ph.D., Department of Cell Science, Institute of Biomedical Science, Fukushima Medical University, Drs. Shizuha Ishiyama and Kaoru Katoh, The National Institute of Advanced Industrial Science and Technology (AIST)<\/p>\n<p>&nbsp;<\/p>\n<h2 class=\"c-heading-b c-accordion-local__section__title__heading\" style=\"font-size: 25px; color: #222222; background-color: #eeeeee;\">\u6d3b\u7ec6\u80de\u6210\u50cf\u7684\u5206\u8fa8\u7387\u662f\u4f20\u7edf\u5149\u5b66\u663e\u5fae\u955c\u7684\u4e24\u500d<\/h2>\n<p>&nbsp;<br \/>\nN-SIM S\u5c06\u5c3c\u5eb7\u7684\u521b\u65b0\u65b9\u6cd5\u5e94\u7528\u4e8e\u7ed3\u6784\u7167\u660e\u663e\u5fae\u955c\u6280\u672f\u3002\u901a\u8fc7\u5c06\u8fd9\u9879\u5f3a\u5927\u7684\u6280\u672f\u4e0e\u5c3c\u5eb7\u8457\u540d\u7684\u7269\u955c\u76f8\u7ed3\u5408\uff0c\u53ef\u5b9e\u73b01.49\u65e0\u4e0e\u4f26\u6bd4\u7684\u6570\u503c\u5b54\u5f84\uff0cN-SIM S\u7684\u7a7a\u95f4\u5206\u8fa8\u7387\u51e0\u4e4e\u662f\u4f20\u7edf\u5149\u5b66\u663e\u5fae\u955c\u7684\u4e24\u500d\uff08\u81f3\u7ea6115nm*\uff09\uff0c\u5b9e\u73b0\u4e86\u7ec6\u80de\u5185\u7684\u7ec6\u5fae\u7ed3\u6784\u53ca\u5176\u4e4b\u95f4\u76f8\u4e92\u4f5c\u7528\u53ef\u89c6\u5316\u3002<\/p>\n<p style=\"font-size: 11px;\">*\u6b64\u503c\u662f\u57283D-SIM\u6a21\u5f0f\u4e0b\u7528488 nm\u6fc0\u5149\u6fc0\u53d1\u7684100 nm\u78c1\u73e0\u7684FWHM\u6d4b\u91cf\u503c\u3002\u5728TIRF-SIM\u6a21\u5f0f\u4e0b\uff0c\u4f7f\u7528\u7531488 nm\u6fc0\u5149\u6fc0\u53d1\u768440 nm\u78c1\u73e0\u53ef\u5b9e\u73b086 nm\u3002<\/p>\n<p>&nbsp;<br \/>\n<img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-E-SIM-image_d72c6b3d69abd785036c6ffd0fcc73d5.jpg\" alt=\"\" width=\"640\" height=\"530\" class=\"alignnone size-full wp-image-1109\" srcset=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-E-SIM-image_d72c6b3d69abd785036c6ffd0fcc73d5.jpg 640w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-E-SIM-image_d72c6b3d69abd785036c6ffd0fcc73d5-300x248.jpg 300w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-E-SIM-image_d72c6b3d69abd785036c6ffd0fcc73d5-600x497.jpg 600w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-E-SIM-image_d72c6b3d69abd785036c6ffd0fcc73d5-193x160.jpg 193w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-E-SIM-image_d72c6b3d69abd785036c6ffd0fcc73d5-242x200.jpg 242w\" sizes=\"auto, (max-width: 640px) 100vw, 640px\" \/><\/p>\n<p style=\"font-size: 9px;\">Super-resolution image (3D-SIM)<\/p>\n<p>&nbsp;<br \/>\n<img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-E-3D-3scalebarConv_d72c6b3d69abd785036c6ffd0fcc73d5.jpg\" alt=\"\" width=\"640\" height=\"530\" class=\"alignnone size-full wp-image-1108\" srcset=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-E-3D-3scalebarConv_d72c6b3d69abd785036c6ffd0fcc73d5.jpg 640w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-E-3D-3scalebarConv_d72c6b3d69abd785036c6ffd0fcc73d5-300x248.jpg 300w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-E-3D-3scalebarConv_d72c6b3d69abd785036c6ffd0fcc73d5-600x497.jpg 600w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-E-3D-3scalebarConv_d72c6b3d69abd785036c6ffd0fcc73d5-193x160.jpg 193w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-E-3D-3scalebarConv_d72c6b3d69abd785036c6ffd0fcc73d5-242x200.jpg 242w\" sizes=\"auto, (max-width: 640px) 100vw, 640px\" \/><\/p>\n<p style=\"font-size: 9px;\">Conventional widefield image<\/p>\n<p style=\"font-size: 9px;\">Microtubules in B16 melanoma cell labeled with YFP<br \/>\nObjective: CFI Apochromat TIRF 100XC Oil (NA 1.49)<br \/>\nImage capturing speed: approximately 1.8 sec\/frame (movie)<br \/>\nReconstruction method: Slice<br \/>\nPhotographed with the cooperation of: Dr. Yasushi Okada, Laboratory for Cell Polarity Regulation, Quantitative Biology Center, RIKEN<\/p>\n<p>&nbsp;<br \/>\n<img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-E-image2_d72c6b3d69abd785036c6ffd0fcc73d5.jpg\" alt=\"\" width=\"640\" height=\"539\" class=\"alignnone size-full wp-image-1111\" srcset=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-E-image2_d72c6b3d69abd785036c6ffd0fcc73d5.jpg 640w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-E-image2_d72c6b3d69abd785036c6ffd0fcc73d5-300x253.jpg 300w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-E-image2_d72c6b3d69abd785036c6ffd0fcc73d5-600x505.jpg 600w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-E-image2_d72c6b3d69abd785036c6ffd0fcc73d5-190x160.jpg 190w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-E-image2_d72c6b3d69abd785036c6ffd0fcc73d5-237x200.jpg 237w\" sizes=\"auto, (max-width: 640px) 100vw, 640px\" \/><\/p>\n<p style=\"font-size: 9px;\">Super-resolution image (3D-SIM)<\/p>\n<p>&nbsp;<br \/>\n<img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-E-conventional-2_d72c6b3d69abd785036c6ffd0fcc73d5.jpg\" alt=\"\" width=\"640\" height=\"539\" class=\"alignnone size-full wp-image-1110\" srcset=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-E-conventional-2_d72c6b3d69abd785036c6ffd0fcc73d5.jpg 640w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-E-conventional-2_d72c6b3d69abd785036c6ffd0fcc73d5-300x253.jpg 300w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-E-conventional-2_d72c6b3d69abd785036c6ffd0fcc73d5-600x505.jpg 600w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-E-conventional-2_d72c6b3d69abd785036c6ffd0fcc73d5-190x160.jpg 190w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-E-conventional-2_d72c6b3d69abd785036c6ffd0fcc73d5-237x200.jpg 237w\" sizes=\"auto, (max-width: 640px) 100vw, 640px\" \/><\/p>\n<p style=\"font-size: 9px;\">Conventional widefield image<\/p>\n<p style=\"font-size: 9px;\">Endoplasmic reticulum (ER) in living HeLa cell labeled with GFP<br \/>\nObjective: CFI Apochromat TIRF 100XC Oil (NA 1.49)<br \/>\nImage capturing speed: approximately 1.5 sec\/frame (movie)<br \/>\nReconstruction method: Slice<br \/>\nPhotographed with the cooperation of: Dr. Ikuo Wada, Institute of Biomedical Sciences, Fukushima Medical University School of Medicine<\/p>\n<p>&nbsp;<\/p>\n<h2 class=\"c-heading-b c-accordion-local__section__title__heading\" style=\"font-size: 25px; color: #222222; background-color: #eeeeee;\">\u81ea\u52a8\u5728\u7167\u660e\u6a21\u5f0f\u4e4b\u95f4\u5207\u6362<\/h2>\n<p>&nbsp;<br \/>\n\u65b0\u5f00\u53d1\u7684\u9ad8\u901f\u7ed3\u6784\u5316\u7167\u660e\u6280\u672f\u53ef\u5b9e\u73b0\u5feb\u901f\u91c7\u96c6\u901f\u7387\uff0c\u7167\u660e\u6a21\u5f0f\u4e4b\u95f4\u7684\u81ea\u52a8\u5207\u6362\u4ee5\u53ca\u9488\u5bf9\u4e0d\u540c\u6ce2\u957f\u548c\u653e\u5927\u500d\u7387\u7684\u7ed3\u6784\u5316\u7167\u660e\u6a21\u5f0f\u7684\u81ea\u52a8\u4f18\u5316\u3002\u8fd9\u79cd\u6269\u5c55\u7684\u81ea\u52a8\u5316\u529f\u80fd\u53ef\u5b9e\u73b0\u5feb\u901f\u76842\u8272TIRF-SIM\u6210\u50cf\uff0c\u4ee5\u53ca\u4e0d\u540cSIM\u6a21\u5f0f\u7684\u591a\u8def\u590d\u7528\u3002\u65e0\u8bba\u662f\u7528\u4e8e\u5355\u6a21\u5f0f\u8fd8\u662f\u591a\u6a21\u5f0f\u6210\u50cf\u5b9e\u9a8c\uff0cN-SIM S\u5747\u63d0\u4f9b\u6613\u4e8e\u4f7f\u7528\u7684\u7b80\u5316\u5de5\u4f5c\u6d41\u7a0b\u3002<br \/>\n&nbsp;<\/p>\n<h2 class=\"c-heading-b c-accordion-local__section__title__heading\" style=\"font-size: 25px; color: #222222; background-color: #eeeeee;\">\u83b7\u5f97\u66f4\u5927\u7684\u89c6\u91ce<\/h2>\n<p>&nbsp;<br \/>\nN-SIM S\u53ef\u4ee5\u83b7\u53d6\u5177\u670966 \u00b5m\u6b63\u65b9\u5f62\u5927\u89c6\u573a\u7684\u8d85\u5206\u8fa8\u7387\u56fe\u50cf\u3002 \u8f83\u5927\u7684\u6210\u50cf\u533a\u57df\u53ef\u4e3a\u53d7\u76ca\u4e8e\u8f83\u5927\u89c6\u91ce\uff08\u4f8b\u5982\u795e\u7ecf\u5143\uff09\u7684\u5e94\u7528\/\u6837\u54c1\u63d0\u4f9b\u975e\u5e38\u9ad8\u7684\u901a\u91cf\uff0c\u4ece\u800c\u51cf\u5c11\u4e86\u83b7\u53d6\u6570\u636e\u6240\u9700\u7684\u65f6\u95f4\u548c\u7cbe\u529b\u3002<br \/>\n&nbsp;<br \/>\n<img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-S-growth-cone_d72c6b3d69abd785036c6ffd0fcc73d5.jpg\" alt=\"\" width=\"616\" height=\"640\" class=\"alignnone size-full wp-image-1112\" srcset=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-S-growth-cone_d72c6b3d69abd785036c6ffd0fcc73d5.jpg 616w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-S-growth-cone_d72c6b3d69abd785036c6ffd0fcc73d5-289x300.jpg 289w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-S-growth-cone_d72c6b3d69abd785036c6ffd0fcc73d5-578x600.jpg 578w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-S-growth-cone_d72c6b3d69abd785036c6ffd0fcc73d5-154x160.jpg 154w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-S-growth-cone_d72c6b3d69abd785036c6ffd0fcc73d5-193x200.jpg 193w\" sizes=\"auto, (max-width: 616px) 100vw, 616px\" \/><\/p>\n<p style=\"font-size: 9px;\">Two-color TIRF-SIM imaging of growth cone of NG108 cell labeled with Alexa Fluor\u00ae 488 for F-actin (green) and Alexa Fluor\u00ae 555 for microtubules (orange)<br \/>\nReconstructed image size: 2048 x 2048 pixels (66 \u03bcm x 66 \u03bcm with a 100X objective)<br \/>\nSample courtesy of: Drs. Shizuha Ishiyama and Kaoru Katoh, The National Institute of Advanced Industrial Science and Technology (AIST)<\/p>\n<p>&nbsp;<\/p>\n<h2 class=\"c-heading-b c-accordion-local__section__title__heading\" style=\"font-size: 25px; color: #222222; background-color: #eeeeee;\">\u591a\u79cd\u89c2\u5bdf\u6a21\u5f0f<\/h2>\n<p>&nbsp;<\/p>\n<h2 class=\"c-heading-b c-accordion-local__section__title__heading\" style=\"font-size: 21px; color: #222222; background-color: #eeeeee;\">TIRF-SIM \/ 2D-SIM\u6a21\u5f0f<\/h2>\n<p>&nbsp;<br \/>\n\u6b64\u6a21\u5f0f\u4ee5\u4ee4\u4eba\u96be\u4ee5\u7f6e\u4fe1\u7684\u5bf9\u6bd4\u5ea6\u9ad8\u901f\u6355\u83b7\u8d85\u5206\u8fa8\u73872D\u56fe\u50cf\u3002TIRF-SIM\u6a21\u5f0f\u4f7f\u5168\u5185\u53cd\u5c04\u8367\u5149\u89c2\u5bdf\u7684\u5206\u8fa8\u7387\u662f\u4f20\u7edfTIRF\u663e\u5fae\u955c\u7684\u4e24\u500d\uff0c\u4ece\u800c\u6709\u52a9\u4e8e\u4eba\u4eec\u66f4\u597d\u5730\u4e86\u89e3\u7ec6\u80de\u8868\u9762\u7684\u5206\u5b50\u76f8\u4e92\u4f5c\u7528\u3002<br \/>\n&nbsp;<br \/>\n<img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/TIRF-SIM-image_ab3ff6800edc21b421940c6f13047851.jpg\" alt=\"\" width=\"640\" height=\"640\" class=\"alignnone size-full wp-image-1114\" srcset=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/TIRF-SIM-image_ab3ff6800edc21b421940c6f13047851.jpg 640w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/TIRF-SIM-image_ab3ff6800edc21b421940c6f13047851-150x150.jpg 150w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/TIRF-SIM-image_ab3ff6800edc21b421940c6f13047851-300x300.jpg 300w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/TIRF-SIM-image_ab3ff6800edc21b421940c6f13047851-600x600.jpg 600w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/TIRF-SIM-image_ab3ff6800edc21b421940c6f13047851-160x160.jpg 160w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/TIRF-SIM-image_ab3ff6800edc21b421940c6f13047851-200x200.jpg 200w\" sizes=\"auto, (max-width: 640px) 100vw, 640px\" \/><\/p>\n<p style=\"font-size: 9px;\">TIRF-SIM image<\/p>\n<p>&nbsp;<br \/>\n<img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/conventional-tirf-image_ab3ff6800edc21b421940c6f13047851.jpg\" alt=\"\" width=\"640\" height=\"640\" class=\"alignnone size-full wp-image-1113\" srcset=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/conventional-tirf-image_ab3ff6800edc21b421940c6f13047851.jpg 640w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/conventional-tirf-image_ab3ff6800edc21b421940c6f13047851-150x150.jpg 150w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/conventional-tirf-image_ab3ff6800edc21b421940c6f13047851-300x300.jpg 300w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/conventional-tirf-image_ab3ff6800edc21b421940c6f13047851-600x600.jpg 600w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/conventional-tirf-image_ab3ff6800edc21b421940c6f13047851-160x160.jpg 160w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/conventional-tirf-image_ab3ff6800edc21b421940c6f13047851-200x200.jpg 200w\" sizes=\"auto, (max-width: 640px) 100vw, 640px\" \/><\/p>\n<p style=\"font-size: 9px;\">Conventional TIRF image<\/p>\n<p style=\"font-size: 9px;\">Plasma membrane of B16 melanoma cell labeled with YFP<br \/>\nObjective: CFI Apochromat TIRF 100XC Oil (NA 1.49)<br \/>\nPhotographed with the cooperation of: Dr. Yasushi Okada, Laboratory for Cell Polarity Regulation, Quantitative Biology Center, RIKEN<\/p>\n<p>&nbsp;<\/p>\n<h2 class=\"c-heading-b c-accordion-local__section__title__heading\" style=\"font-size: 21px; color: #222222; background-color: #eeeeee;\">3D-SIM\u6a21\u5f0f<\/h2>\n<p>&nbsp;<br \/>\n3D-SIM\u6a21\u5f0f\u53ef\u751f\u6210\u4e09\u7ef4\u7ed3\u6784\u5316\u7167\u660e\u56fe\u6848\uff0c\u4ece\u800c\u5728\u6a2a\u5411\u548c\u8f74\u5411\u5206\u8fa8\u7387\u4e0a\u5b9e\u73b0\u4e24\u500d\u7684\u6539\u8fdb\u3002 \u6709\u4e24\u79cd\u91cd\u5efa\u65b9\u6cd5\uff08\u201c\u5207\u7247\u201d\u548c\u201c\u5806\u6808\u201d\uff09\u53ef\u6839\u636e\u5e94\u7528\u8981\u6c42\uff08\u4f8b\u5982\u6837\u54c1\u539a\u5ea6\uff0c\u901f\u5ea6\u7b49\uff09\u6765\u4f18\u5316\u7ed3\u679c\u3002 \u5207\u7247\u91cd\u5efa\u9002\u7528\u4e8e\u5728\u7279\u5b9a\u6df1\u5ea6\u7684\u6d3b\u7ec6\u80de\u6210\u50cf\uff0c\u56e0\u4e3a\u5b83\u5141\u8bb8\u4ee5300nm\u5206\u8fa8\u7387\u7684\u5149\u5b66\u5207\u7247\u8fdb\u884c\u8f74\u5411\u8d85\u5206\u8fa8\u7387\u6210\u50cf\u3002\u6839\u636e\u53e4\u65af\u5854\u592b\u68ee\uff08Gustafsson\uff09\u7684\u7406\u8bba\uff0c\u5806\u6808\u91cd\u5efa\u6280\u672f\u9002\u7528\u4e8e\u91c7\u96c6\u4f53\u79ef\u6570\u636e\uff0c\u56e0\u4e3a\u5b83\u53ef\u4ee5\u6bd4\u5207\u7247\u91cd\u5efa\u6280\u672f\u4ee5\u66f4\u9ad8\u7684\u5bf9\u6bd4\u5ea6\u6210\u50cf\u66f4\u539a\u7684\u6807\u672c\u3002<br \/>\n&nbsp;<br \/>\n<img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-E-bacillus1_ab3ff6800edc21b421940c6f13047851.jpg\" alt=\"\" width=\"640\" height=\"452\" class=\"alignnone size-full wp-image-1115\" srcset=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-E-bacillus1_ab3ff6800edc21b421940c6f13047851.jpg 640w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-E-bacillus1_ab3ff6800edc21b421940c6f13047851-300x212.jpg 300w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-E-bacillus1_ab3ff6800edc21b421940c6f13047851-600x424.jpg 600w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-E-bacillus1_ab3ff6800edc21b421940c6f13047851-227x160.jpg 227w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-E-bacillus1_ab3ff6800edc21b421940c6f13047851-283x200.jpg 283w\" sizes=\"auto, (max-width: 640px) 100vw, 640px\" \/><\/p>\n<p style=\"font-size: 9px;\">3D-SIM image<\/p>\n<p>&nbsp;<br \/>\n<img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-E-bacillus2_ab3ff6800edc21b421940c6f13047851.jpg\" alt=\"\" width=\"640\" height=\"452\" class=\"alignnone size-full wp-image-1116\" srcset=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-E-bacillus2_ab3ff6800edc21b421940c6f13047851.jpg 640w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-E-bacillus2_ab3ff6800edc21b421940c6f13047851-300x212.jpg 300w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-E-bacillus2_ab3ff6800edc21b421940c6f13047851-600x424.jpg 600w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-E-bacillus2_ab3ff6800edc21b421940c6f13047851-227x160.jpg 227w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-E-bacillus2_ab3ff6800edc21b421940c6f13047851-283x200.jpg 283w\" sizes=\"auto, (max-width: 640px) 100vw, 640px\" \/><\/p>\n<p style=\"font-size: 9px;\">Conventional widefield image<\/p>\n<p style=\"font-size: 9px;\">Bacillus subtilis bacterium stained with membrane dye Nile Red (red), and expressing the cell division protein DivIVA fused to GFP (green). The super-resolution microscope enables accurate localization of the protein during division.<br \/>\nReconstruction method: Slice<br \/>\nImages courtesy of: Drs. Henrik Strahl and Leendert Hamoen, Centre for Bacterial Cell Biology, Newcastle University<\/p>\n<p>&nbsp;<br \/>\n<img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-E-keratinocyte1_ab3ff6800edc21b421940c6f13047851.jpg\" alt=\"\" width=\"640\" height=\"618\" class=\"alignnone size-full wp-image-1117\" srcset=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-E-keratinocyte1_ab3ff6800edc21b421940c6f13047851.jpg 640w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-E-keratinocyte1_ab3ff6800edc21b421940c6f13047851-300x290.jpg 300w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-E-keratinocyte1_ab3ff6800edc21b421940c6f13047851-600x579.jpg 600w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-E-keratinocyte1_ab3ff6800edc21b421940c6f13047851-166x160.jpg 166w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-E-keratinocyte1_ab3ff6800edc21b421940c6f13047851-207x200.jpg 207w\" sizes=\"auto, (max-width: 640px) 100vw, 640px\" \/><\/p>\n<p style=\"font-size: 9px;\">3D-SIM (Volume view)<br \/>\nWidth: 26.16 \u03bcm, Height: 27.11 \u03bcm, Depth: 3.36 \u03bcm<\/p>\n<p>&nbsp;<br \/>\n<img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-E-keratinocyte2_ab3ff6800edc21b421940c6f13047851.jpg\" alt=\"\" width=\"619\" height=\"640\" class=\"alignnone size-full wp-image-1118\" srcset=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-E-keratinocyte2_ab3ff6800edc21b421940c6f13047851.jpg 619w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-E-keratinocyte2_ab3ff6800edc21b421940c6f13047851-290x300.jpg 290w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-E-keratinocyte2_ab3ff6800edc21b421940c6f13047851-580x600.jpg 580w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-E-keratinocyte2_ab3ff6800edc21b421940c6f13047851-155x160.jpg 155w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-E-keratinocyte2_ab3ff6800edc21b421940c6f13047851-193x200.jpg 193w\" sizes=\"auto, (max-width: 619px) 100vw, 619px\" \/><\/p>\n<p style=\"font-size: 9px;\">3D-SIM (Maximum projection)<\/p>\n<p style=\"font-size: 9px;\">Mouse keratinocyte indirectly immunolabeled for keratin intermediate filaments and visualized with Alexa Fluor\u00ae 488 conjugated secondary antibodies.<br \/>\nReconstruction method: Stack<br \/>\nImage courtesy of: Dr. Reinhard Windoffer, RWTH Aachen University<\/p>\n<p>&nbsp;<br \/>\n<img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-S-fixed-cultured-neuron-SIM_ab3ff6800edc21b421940c6f13047851.jpg\" alt=\"\" width=\"640\" height=\"615\" class=\"alignnone size-full wp-image-1120\" srcset=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-S-fixed-cultured-neuron-SIM_ab3ff6800edc21b421940c6f13047851.jpg 640w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-S-fixed-cultured-neuron-SIM_ab3ff6800edc21b421940c6f13047851-300x288.jpg 300w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-S-fixed-cultured-neuron-SIM_ab3ff6800edc21b421940c6f13047851-600x577.jpg 600w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-S-fixed-cultured-neuron-SIM_ab3ff6800edc21b421940c6f13047851-167x160.jpg 167w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-S-fixed-cultured-neuron-SIM_ab3ff6800edc21b421940c6f13047851-208x200.jpg 208w\" sizes=\"auto, (max-width: 640px) 100vw, 640px\" \/><\/p>\n<p style=\"font-size: 9px;\">N-SIM S image<br \/>\nZ-stack of 3D-SIM ,19 stps, Z range: 2 \u03bcm<\/p>\n<p>&nbsp;<br \/>\n<img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-S-fixed-cultured-neuron-Decon_ab3ff6800edc21b421940c6f13047851.jpg\" alt=\"\" width=\"640\" height=\"615\" class=\"alignnone size-full wp-image-1119\" srcset=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-S-fixed-cultured-neuron-Decon_ab3ff6800edc21b421940c6f13047851.jpg 640w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-S-fixed-cultured-neuron-Decon_ab3ff6800edc21b421940c6f13047851-300x288.jpg 300w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-S-fixed-cultured-neuron-Decon_ab3ff6800edc21b421940c6f13047851-600x577.jpg 600w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-S-fixed-cultured-neuron-Decon_ab3ff6800edc21b421940c6f13047851-167x160.jpg 167w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-S-fixed-cultured-neuron-Decon_ab3ff6800edc21b421940c6f13047851-208x200.jpg 208w\" sizes=\"auto, (max-width: 640px) 100vw, 640px\" \/><\/p>\n<p style=\"font-size: 9px;\">Confocal A1R image with 0.4AU Deconvolution<br \/>\nZ-stack ,19 stps, Z range: 2 \u03bcm<\/p>\n<p style=\"font-size: 9px;\">Sample information: Dendritic spine in mouse hippocampal neuron expressing GFP<br \/>\nImage courtesy of: Drs. Yutaro Kashiwagi and Shigeo Okabe, Department of Cellular Neurobiology, Graduate School of Medicine and Faculty of Medicine, The University of Tokyo.<\/p>\n<p>&nbsp;<br \/>\n<div style=\"width: 800px;\" class=\"wp-video\"><video class=\"wp-video-shortcode\" id=\"video-1097-6\" width=\"800\" height=\"540\" preload=\"metadata\" controls=\"controls\"><source type=\"video\/mp4\" src=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/20200228083705-105.mp4?_=6\" \/><a href=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/20200228083705-105.mp4\">http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/20200228083705-105.mp4<\/a><\/video><\/div><\/p>\n<p style=\"font-size: 9px;\">Quantitative spine analysis with Z-stack of 3D-SIM, 35 stps, Z range: 4.2 um<br \/>\nExposure time 100 msec, 120 sec interval<br \/>\nTime-lapse of 11 times<br \/>\nExcitation wavelength 488 nm<\/p>\n<p style=\"font-size: 9px;\">Sample information: Dendritic spine in mouse hippocampal neuron expressing GFP<br \/>\nMovie courtesy of: Drs. Yutaro Kashiwagi and Shigeo Okabe, Department of Cellular Neurobiology, Graduate School of Medicine and Faculty of Medicine, The University of Tokyo.<\/p>\n<p>&nbsp;<\/p>\n<h2 class=\"c-heading-b c-accordion-local__section__title__heading\" style=\"font-size: 25px; color: #222222; background-color: #eeeeee;\">\u4e24\u901a\u9053\u540c\u65f6\u6210\u50cf\uff08\u9009\u914d\uff09<\/h2>\n<p>&nbsp;<br \/>\n\u901a\u8fc7\u4f7f\u7528\u53ef\u9009\u7684\u53cc\u76f8\u673a\u6210\u50cf\u9002\u914d\u5668*\u548c\u4e24\u53f0sCMOS\u6444\u50cf\u673a\uff0c\u53ef\u4ee5\u540c\u65f6\u8fdb\u884c\u53cc\u8272\u6210\u50cf\u5b9e\u9a8c\u3002<\/p>\n<p style=\"font-size: 11px;\">*\u7531\u6ee8\u677e\u5149\u5b50\u63d0\u4f9b<\/p>\n<p>&nbsp;<br \/>\n<div style=\"width: 993px;\" class=\"wp-video\"><video class=\"wp-video-shortcode\" id=\"video-1097-7\" width=\"993\" height=\"248\" preload=\"metadata\" controls=\"controls\"><source type=\"video\/mp4\" src=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/vlc-output2.mp4?_=7\" \/><a href=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/vlc-output2.mp4\">http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/vlc-output2.mp4<\/a><\/video><\/div><\/p>\n<p style=\"font-size: 9px;\">Immediately before drug application&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;10 minutes after&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;20 minutes after&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;30 minutes after<\/p>\n<p style=\"font-size: 9px;\">Growth cone of NG108 cells. \u3000Fascin (green): GFP-Fascin, Actin (red): LifeAct-KO\u3000 Objective Lens : CFI SR HP Apochromat TIRF 100xC oil<\/p>\n<p style=\"font-size: 9px;\">Using image splitting optical system, dual color time-lapse images were captured after application of drug solution. The time course was recorded that fascin was dissociated from the actin bundles and filamentous actin changed their pattern. Co-localization of fascin and actin and dissociation of fascin were well demonstrated. Optic aberrations and image distortion were adjusted to be less than 30 nm.<\/p>\n<p style=\"font-size: 9px;\">Movie courtesy of: Dr. Minami Tanaka, Dr. Kaoru Katoh, Biomedical Research Institute Molecular Neurobiology Group, National Institute of Advanced Industrial Science and Technology<\/p>\n<p>&nbsp;<\/p>\n<h2 class=\"c-heading-b c-accordion-local__section__title__heading\" style=\"font-size: 25px; color: #222222; background-color: #eeeeee;\">\u5728\u6210\u50cf\u6a21\u5f0f\u4e4b\u95f4\u65e0\u7f1d\u5207\u6362\uff0c\u4ee5\u8fdb\u884c\u591a\u9636\u5b9e\u9a8c<\/h2>\n<p>&nbsp;<br \/>\nN-SIM S\u53ef\u4ee5\u4e0e\u5171\u805a\u7126\u663e\u5fae\u955c\uff08\u4f8b\u5982A1 HD25\uff09\u8fdb\u884c\u7ec4\u5408\u3002\u53ef\u4ee5\u5728\u4f4e\u500d\u7387\/\u5927\u89c6\u91ce\u7684\u5171\u805a\u7126\u56fe\u50cf\u4e2d\u6307\u5b9a\u6837\u672c\u4e2d\u7684\u6240\u9700\u4f4d\u7f6e\uff0c\u5e76\u53ea\u9700\u5207\u6362\u6210\u50cf\u65b9\u6cd5\u5373\u53ef\u4ee5\u83b7\u53d6\u8d85\u5206\u8fa8\u7387\u5c40\u90e8\u56fe\u50cf\u3002\u5c06\u5171\u805a\u7126\u663e\u5fae\u955c\u4e0e\u8d85\u5206\u8fa8\u7387\u7cfb\u7edf\u7ed3\u5408\u4f7f\u7528\u53ef\u4ee5\u63d0\u4f9b\u4e00\u79cd\u5c06\u8d85\u5206\u8fa8\u7387\u4fe1\u606f\u7684\u589e\u5f3a\u5230\u8f83\u4ea4\u5927\u5c3a\u5ea6\u89c6\u56fe\u7684\u65b9\u6cd5\u3002<br \/>\n&nbsp;<br \/>\n<img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-E-confocal-ROI_ab3ff6800edc21b421940c6f13047851.jpg\" alt=\"\" width=\"640\" height=\"640\" class=\"alignnone size-full wp-image-1121\" srcset=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-E-confocal-ROI_ab3ff6800edc21b421940c6f13047851.jpg 640w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-E-confocal-ROI_ab3ff6800edc21b421940c6f13047851-150x150.jpg 150w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-E-confocal-ROI_ab3ff6800edc21b421940c6f13047851-300x300.jpg 300w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-E-confocal-ROI_ab3ff6800edc21b421940c6f13047851-600x600.jpg 600w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-E-confocal-ROI_ab3ff6800edc21b421940c6f13047851-160x160.jpg 160w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-SIM-E-confocal-ROI_ab3ff6800edc21b421940c6f13047851-200x200.jpg 200w\" sizes=\"auto, (max-width: 640px) 100vw, 640px\" \/><\/p>\n<p style=\"font-size: 9px;\">Select the location to acquire a SIM image in a confocal image<\/p>\n<p>&nbsp;<br \/>\n<img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/SIM-E-super-res-detail_ab3ff6800edc21b421940c6f13047851.jpg\" alt=\"\" width=\"640\" height=\"640\" class=\"alignnone size-full wp-image-1122\" srcset=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/SIM-E-super-res-detail_ab3ff6800edc21b421940c6f13047851.jpg 640w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/SIM-E-super-res-detail_ab3ff6800edc21b421940c6f13047851-150x150.jpg 150w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/SIM-E-super-res-detail_ab3ff6800edc21b421940c6f13047851-300x300.jpg 300w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/SIM-E-super-res-detail_ab3ff6800edc21b421940c6f13047851-600x600.jpg 600w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/SIM-E-super-res-detail_ab3ff6800edc21b421940c6f13047851-160x160.jpg 160w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/SIM-E-super-res-detail_ab3ff6800edc21b421940c6f13047851-200x200.jpg 200w\" sizes=\"auto, (max-width: 640px) 100vw, 640px\" \/><\/p>\n<p style=\"font-size: 9px;\">Acquire the SIM image of the selected location<\/p>\n<p>&nbsp;<\/p>\n<h2 class=\"c-heading-b c-accordion-local__section__title__heading\" style=\"font-size: 25px; color: #222222; background-color: #eeeeee;\">\u8d85\u9ad8\u5206\u8fa8\u7387\u663e\u5fae\u955c\u4e13\u7528\u7269\u955c<\/h2>\n<p>&nbsp;<\/p>\n<h2 class=\"c-heading-b c-accordion-local__section__title__heading\" style=\"font-size: 21px; color: #222222; background-color: #eeeeee;\">\u7845\u6cb9\u7269\u955c<\/h2>\n<p>&nbsp;<br \/>\n\u7845\u6cb9\u6d78\u5165\u7269\u955c\u4f7f\u7528\u63a5\u8fd1\u6d3b\u7ec6\u80de\u6298\u5c04\u7387\u7684\u9ad8\u7c98\u5ea6\u7845\u6cb9\u4f5c\u4e3a\u6d78\u5165\u6db2\u3002\u7531\u4e8e\u6539\u5584\u4e86\u6298\u5c04\u7387\u7684\u517c\u5bb9\u6027\uff0c\u56e0\u6b64\u5f53\u5728\u66f4\u6df1\u7684\u6837\u54c1\u4e2d\u6267\u884c\u8d85\u5206\u8fa8\u7387\u6210\u50cf\u65f6\uff0c\u8fd9\u4e9b\u7269\u955c\u53ef\u4ee5\u63d0\u4f9b\u66f4\u9ad8\u7684\u5149\u5b50\u6536\u96c6\u80fd\u529b\u548c\u5206\u8fa8\u7387\u3002 \u5b83\u4eec\u5728\u5f88\u5bbd\u7684\u6ce2\u957f\u8303\u56f4\u5185\u5747\u5177\u6709\u51fa\u8272\u7684\u8272\u5dee\u6821\u6b63\u548c\u9ad8\u900f\u5c04\u7387\u3002<br \/>\n&nbsp;<br \/>\n<div style=\"width: 660px;\" class=\"wp-video\"><video class=\"wp-video-shortcode\" id=\"video-1097-8\" width=\"660\" height=\"360\" preload=\"metadata\" controls=\"controls\"><source type=\"video\/mp4\" src=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/20200228084054-801.mp4?_=8\" \/><a href=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/20200228084054-801.mp4\">http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/20200228084054-801.mp4<\/a><\/video><\/div><\/p>\n<p style=\"font-size: 9px;\">Mouse brain section labeled with tdTomato expressing neurons<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/CFI-SR-HP-Plan-Apo_d72c6b3d69abd785036c6ffd0fcc73d5.jpg\" alt=\"\" width=\"366\" height=\"640\" class=\"alignnone size-full wp-image-1123\" srcset=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/CFI-SR-HP-Plan-Apo_d72c6b3d69abd785036c6ffd0fcc73d5.jpg 366w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/CFI-SR-HP-Plan-Apo_d72c6b3d69abd785036c6ffd0fcc73d5-172x300.jpg 172w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/CFI-SR-HP-Plan-Apo_d72c6b3d69abd785036c6ffd0fcc73d5-343x600.jpg 343w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/CFI-SR-HP-Plan-Apo_d72c6b3d69abd785036c6ffd0fcc73d5-92x160.jpg 92w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/CFI-SR-HP-Plan-Apo_d72c6b3d69abd785036c6ffd0fcc73d5-114x200.jpg 114w\" sizes=\"auto, (max-width: 366px) 100vw, 366px\" \/><\/p>\n<p style=\"font-size: 9px;\">CFI SR HP Plan Apochromat Lambda S 100XC Sil<\/p>\n<p>&nbsp;<\/p>\n<h2 class=\"c-heading-b c-accordion-local__section__title__heading\" style=\"font-size: 21px; color: #222222; background-color: #eeeeee;\">\u6d78\u5165\u7269\u955c<\/h2>\n<p>&nbsp;<br \/>\nSR\u7cfb\u5217\u7269\u955c\u4f7f\u7528\u6ce2\u524d\u50cf\u5dee\u6d4b\u91cf\u6280\u672f\u8fdb\u884c\u5bf9\u51c6\u548c\u68c0\u67e5\uff0c\u4ee5\u5c3d\u53ef\u80fd\u964d\u4f4e\u975e\u5bf9\u79f0\u50cf\u5dee\u548c\u786e\u4fdd\u8d85\u5206\u8fa8\u7387\u6210\u50cf\u6240\u9700\u7684\u51fa\u8272\u5149\u5b66\u6027\u80fd\u3002HP\u578b\u53f7\u7269\u955c\u53ef\u63d0\u4f9b\u8d85\u9ad8\u529f\u7387\u6fc0\u5149\u6fc0\u53d1\u7684\u8010\u4e45\u6027\uff0c\u5e76\u6539\u5584\u4e86\u8f74\u5411\u8272\u5dee\u6821\u6b63\uff0c\u4ece\u800c\u65e0\u9700\u5728N-SIM S\u548cN-STORM\u7cfb\u7edf\u4e4b\u95f4\u5207\u6362\u7269\u955c\u3002\u652f\u6301Ti2-E\u663e\u5fae\u955c\u7684\u81ea\u52a8\u6821\u6b63\u73af\u7684AC\u578b\u7269\u955c\u53ef\u5b9e\u73b0\u5bf9\u6821\u6b63\u73af\u7684\u7cbe\u786e\u800c\u8f7b\u677e\u7684\u8c03\u6574\u3002<br \/>\n&nbsp;<br \/>\n<img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-STORM-SR-100XC-Oil-2_d814e09a934119d51c08e8d9a606b542.jpg\" alt=\"\" width=\"368\" height=\"426\" class=\"alignnone size-full wp-image-1126\" srcset=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-STORM-SR-100XC-Oil-2_d814e09a934119d51c08e8d9a606b542.jpg 368w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-STORM-SR-100XC-Oil-2_d814e09a934119d51c08e8d9a606b542-259x300.jpg 259w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-STORM-SR-100XC-Oil-2_d814e09a934119d51c08e8d9a606b542-138x160.jpg 138w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-STORM-SR-100XC-Oil-2_d814e09a934119d51c08e8d9a606b542-173x200.jpg 173w\" sizes=\"auto, (max-width: 368px) 100vw, 368px\" \/><\/p>\n<p style=\"font-size: 9px;\">CFI SR HP Apochromat TIRF 100XC Oil<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-STORM-100XC-Oil_d814e09a934119d51c08e8d9a606b542.jpg\" alt=\"\" width=\"392\" height=\"426\" class=\"alignnone size-full wp-image-1124\" srcset=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-STORM-100XC-Oil_d814e09a934119d51c08e8d9a606b542.jpg 392w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-STORM-100XC-Oil_d814e09a934119d51c08e8d9a606b542-276x300.jpg 276w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-STORM-100XC-Oil_d814e09a934119d51c08e8d9a606b542-147x160.jpg 147w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-STORM-100XC-Oil_d814e09a934119d51c08e8d9a606b542-184x200.jpg 184w\" sizes=\"auto, (max-width: 392px) 100vw, 392px\" \/><\/p>\n<p style=\"font-size: 9px;\">CFI SR Plan Apochromat IR 60XC WI<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-STORM-SR-60X-WI_d814e09a934119d51c08e8d9a606b542.jpg\" alt=\"\" width=\"368\" height=\"426\" class=\"alignnone size-full wp-image-1125\" srcset=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-STORM-SR-60X-WI_d814e09a934119d51c08e8d9a606b542.jpg 368w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-STORM-SR-60X-WI_d814e09a934119d51c08e8d9a606b542-259x300.jpg 259w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-STORM-SR-60X-WI_d814e09a934119d51c08e8d9a606b542-138x160.jpg 138w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/N-STORM-SR-60X-WI_d814e09a934119d51c08e8d9a606b542-173x200.jpg 173w\" sizes=\"auto, (max-width: 368px) 100vw, 368px\" \/><\/p>\n<p style=\"font-size: 9px;\">CFI SR Plan Apochromat IR 60XC WI<\/p>\n<p>&nbsp;<\/p>\n<h2 class=\"c-heading-b c-accordion-local__section__title__heading\" style=\"font-size: 21px; color: #222222; background-color: #eeeeee;\">\u5e72\u5f0f\u7269\u955c<\/h2>\n<p>&nbsp;<br \/>\nN-SIM S\u4e0e\u5e72\u5f0f\u7269\u955c\u517c\u5bb9\uff0c\u65e0\u9700\u5207\u6362\u955c\u5934\u5373\u53ef\u4f7f\u7528\u8d85\u5206\u8fa8\u7387\u6210\u50cf\u548c\u5171\u805a\u7126\u6210\u50cf\u3002\u4f4e\u500d\u7387\uff0c\u5bbd\u89c6\u573a\u7684\u5e72\u5f0f\u7269\u955c\u5373\u4f7f\u5728\u6837\u54c1\u7ec4\u7ec7\u7684\u5916\u56f4\u4e5f\u80fd\u8fdb\u884c\u9ad8\u5206\u8fa8\u7387\u89c2\u5bdf\u3002<\/p>\n<p style=\"font-size: 11px;\">*\u5e72\u5f0f\u7269\u955c\u652f\u63012D-SIM\u548c3D-SIM\uff08\u5207\u7247\u91cd\u5efa\uff09<\/p>\n<p>&nbsp;<br \/>\n<img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/CFI-Plan-Apo-Lambda-60X-0.95_d72c6b3d69abd785036c6ffd0fcc73d5.jpg\" alt=\"\" width=\"552\" height=\"640\" class=\"alignnone size-full wp-image-1128\" srcset=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/CFI-Plan-Apo-Lambda-60X-0.95_d72c6b3d69abd785036c6ffd0fcc73d5.jpg 552w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/CFI-Plan-Apo-Lambda-60X-0.95_d72c6b3d69abd785036c6ffd0fcc73d5-259x300.jpg 259w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/CFI-Plan-Apo-Lambda-60X-0.95_d72c6b3d69abd785036c6ffd0fcc73d5-518x600.jpg 518w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/CFI-Plan-Apo-Lambda-60X-0.95_d72c6b3d69abd785036c6ffd0fcc73d5-138x160.jpg 138w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/CFI-Plan-Apo-Lambda-60X-0.95_d72c6b3d69abd785036c6ffd0fcc73d5-173x200.jpg 173w\" sizes=\"auto, (max-width: 552px) 100vw, 552px\" \/>&nbsp;&nbsp;<img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/CFI-Plan-Apo-Lambda-40X-0.95_d72c6b3d69abd785036c6ffd0fcc73d5.jpg\" alt=\"\" width=\"552\" height=\"640\" class=\"alignnone size-full wp-image-1127\" srcset=\"http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/CFI-Plan-Apo-Lambda-40X-0.95_d72c6b3d69abd785036c6ffd0fcc73d5.jpg 552w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/CFI-Plan-Apo-Lambda-40X-0.95_d72c6b3d69abd785036c6ffd0fcc73d5-259x300.jpg 259w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/CFI-Plan-Apo-Lambda-40X-0.95_d72c6b3d69abd785036c6ffd0fcc73d5-518x600.jpg 518w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/CFI-Plan-Apo-Lambda-40X-0.95_d72c6b3d69abd785036c6ffd0fcc73d5-138x160.jpg 138w, http:\/\/www.tangning.net\/wordpress\/wp-content\/uploads\/2020\/02\/CFI-Plan-Apo-Lambda-40X-0.95_d72c6b3d69abd785036c6ffd0fcc73d5-173x200.jpg 173w\" sizes=\"auto, (max-width: 552px) 100vw, 552px\" \/><\/p>\n","protected":false},"excerpt":{"rendered":"<h2 class=\"c-heading-b c-accordion-local__section__title__heading\" style=\"font-size: 25px; 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