超大画幅高灵敏度单色荧光相机DS-Qi2

捕获低亮度荧光和大视野图像

高分辨率、高灵敏度和低噪声是DS-Qi2单色相机的关键特征。

Description

超大尺寸CMOS传感器

尼康为专业高端单反相机设计、生产的CMOS传感器和成像技术,现在已经为显微镜成像进行了优化。


 

高灵敏度

 

可探测微弱的荧光信号

7.3μm像素尺寸,高量子效率QE和非常低的读取噪声,允许DS-Qi2甚至探测非常微弱的荧光信号。

Indian Muntjac Deer Skin Fibroblast Cells, Cytoskeletal F-actin labeled with Alexa Fluor 488

Sample courtesy of: Michael Davidson and Florida State University


 

出色的线性表达

 

可靠的定量分析成为可能

DS-Qi2线性误差为±1%,是测量荧光样品强度的绝佳工具,包括基于时间的强度测量和比例测量。


 

高帧速率

 

即使荧光图像也可快速对焦

2.2个电子读出噪声配合大容量像素及0.6电子暗电流使其可以拍摄极小噪声的荧光图像。


 

低噪声

 

获取微弱荧光的极低噪声图像

2.2个电子读出噪声配合大容量像素,使获取的荧光图像具有极低的噪声和极高的动态范围。
    

LLC-PK1 cells expressing GFP-EB3 tubulin Low noise and large linear full well capacity allows the acquisition of large dynamic range in a single capture.

A captured image is shown intensity scaled to show both the brightest and dimmest areas.

Sample courtesy of: Michael Davidson, National High Magnetic Field Laboratory, Florida State University


 

时间序列成像

 

使用NIS-Elements软件进行荧光时间序列成像

结合宽视野和高像素密度及低噪声,DS-Qi2是时间序列拍摄应用的绝佳选择。

Time-lapse images (every 1 second) of LLC-PK1 cells with GFP-EB3 tubulin. Each image represents the maximum intensity projection of the timelapse, allowing visualization of the end-binding protein located on the microtubule plus-ends, and allowing tracing of the microtubule path.

DS-Qi2 captures an extremely large field of view, but still represents very fine details as demonstrated in this cropped timelapse sequence from a large FOV image.

Objective: CFI Plan Apochromat Lambda 60X Oil (NA: 1.4)

Sample courtesy of: Michael Davidson, National High Magnetic Field Laboratory, Florida State University